Fraxin inhibits lipopolysaccharide-induced inflammatory cytokines and protects against endotoxic shock in mice.

Abstract

Fraxin, the effective component isolated from Cortex Fraxini, has been reported to have anti-inflammation effects. The aim of this study was to explore the effect of fraxin on lipopolysaccharide (LPS)-induced endotoxic shock in mice. We used Kunming male mice to establish the model, and we found that fraxin could improve the survival rate of the LPS-induced mice. Histopathological study showed that fraxin could mitigate the injuries in LPS-induced lung and liver tissues. The levels of tumour necrosis factor-α and interleukin-6 both in serum and lung, liver tissues, and the productions of nitric oxide (NO), aspartate transaminase and alanine transaminase in serum were decreased by fraxin. Western blot assay demonstrated that the pretreatment with fraxin could downregulate LPS-induced protein expressions of nuclear factor-kappa B (NF-κB) and NLRP3 inflammatory corpuscle signalling pathways. Overall, fraxin had protective effects on LPS-induced endotoxic shock mice and the possible mechanisms might activate through NF-κB and NLRP3 inflammatory corpuscle signalling pathways.

• Authors: Weifeng Li, Wenqi Li, JinJin Yu, Fang Liu, Lulu Zang, Xin Xiao, Jinmeng Zhao, Qing Yao, Xiaofeng Niu
• Journal: Fundamental & clinical pharmacology (Fundam Clin Pharmacol) Vol. 34 Issue 1 Pg. 91-101 (Feb 2020) ISSN: 1472-8206 [Electronic] England
• PMID: 31325387 (Publication Type: Journal Article)
• Copyright: © 2019 Société Française de Pharmacologie et de Thérapeutique.

Chemical References

• Anti-Inflammatory Agents
• Coumarins
• Cytokines
• Lipopolysaccharides
• NF-kappa B
• NLR Family, Pyrin Domain-Containing 3 Protein
• Nlrp3 protein, mouse
• Nitric Oxide
• fraxin

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Coumarins (pharmacology)
• Cytokines (metabolism)
• Disease Models, Animal
• Inflammation (drug therapy, pathology)
• Lipopolysaccharides (toxicity)
• Male
• Mice
• NF-kappa B (metabolism)
• NLR Family, Pyrin Domain-Containing 3 Protein (metabolism)
• Nitric Oxide (metabolism)
• Shock, Septic (prevention & control)
• Signal Transduction (drug effects)
• Survival Rate