Abstract | BACKGROUND: METHODS: RESULTS: UCH-L1 levels were not significantly different between TBI, tail injury, and sham TBI. By contrast, neuron-specific enolase levels were increased in TBI mice compared with tail injury and sham TBI mice. UCH-L1 levels increased regardless of TBI status at 30 min and 4 h after hemorrhagic shock and resuscitation. In mice that underwent femoral artery cannulation followed by hemorrhagic shock/ resuscitation, UCH-L1 levels were significantly elevated compared with shock sham mice at 4 h (3158 ± 2168 pg/mL, 4 h shock versus 0 ± 0 pg/mL, 4 h shock sham; P < 0.01) and at 24 h (3253 ± 2954 pg/mL, 24 h shock versus 324 ± 482 pg/mL, 24 h shock sham; P = 0.03). No differences were observed in UCH-L1 levels between the sham shock and the arterial ligation, vein ligation, or extremity ischemia/reperfusion groups at any time point. Similar to UCH-L1, creatine kinase was elevated only after shock compared with sham mice at 4, 24, and 72 h after injury. CONCLUSIONS: Our study demonstrates that UCH-L1 is not a specific marker for TBI but is elevated in models that induce central and peripheral nerve ischemia. Given the increase in UCH-L1 levels observed after hemorrhagic shock, we propose that UCH-L1 may be a useful adjunct in quantifying severity of shock or global ischemia rather than as a specific marker of TBI.
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Authors | Mackenzie C Morris, Aron Bercz, Grace M Niziolek, Farzaan Kassam, Rose Veile, Lou Ann Friend, Timothy A Pritts, Amy T Makley, Michael D Goodman |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 244
Pg. 63-68
(12 2019)
ISSN: 1095-8673 [Electronic] United States |
PMID | 31279265
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | Copyright © 2019 Elsevier Inc. All rights reserved. |
Chemical References |
- Biomarkers
- Ubiquitin carboxyl-Terminal Hydrolase L-1, mouse
- Ubiquitin Thiolesterase
|
Topics |
- Animals
- Biomarkers
(blood)
- Brain Injuries, Traumatic
(blood, diagnosis, etiology)
- Disease Models, Animal
- Glasgow Coma Scale
- Humans
- Male
- Mice
- Multiple Trauma
(blood, complications)
- Severity of Illness Index
- Shock, Hemorrhagic
(blood, diagnosis, etiology)
- Ubiquitin Thiolesterase
(blood)
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