Abstract | BACKGROUND: METHODS: Near-term fetal sheep were randomized to sham control or cerebral ischemia for 30 min with normothermia or cerebral hypothermia from 3 to either 48 or 72 h. Sheep were killed after 7 days. SMI-312-labeled axons and myelin basic protein were quantified in the intragyral white matter of the first and second parasagittal gyri. RESULTS:
Ischemia was associated with reduced axonal and myelin area fraction (p < 0.05); loss of axonal and myelin linearity (p < 0.05); and thin, sparse axons, with spheroids, compared to dense, linear morphology in sham controls and associated with induction of microglia in an amoeboid morphology. Both ischemia-48 h hypothermia and ischemia-72 h hypothermia improved axonal area fraction and linearity (p < 0.05), although abnormal morphological features were seen in a subset. Microglial induction was partially suppressed by ischemia-48 h hypothermia, with a ramified morphology. CONCLUSIONS:
|
Authors | Kelly Q Zhou, Vittoria Draghi, Christopher A Lear, Justin M Dean, Jesse L Ashton, Yufeng Hou, Laura Bennet, Alistair J Gunn, Joanne O Davidson |
Journal | Pediatric research
(Pediatr Res)
Vol. 88
Issue 1
Pg. 48-56
(07 2020)
ISSN: 1530-0447 [Electronic] United States |
PMID | 31234193
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Topics |
- Animals
- Axons
(pathology, physiology)
- Blood Gas Analysis
- Brain
(embryology, physiopathology)
- Brain Ischemia
(physiopathology)
- Electroencephalography
- Fetus
(metabolism)
- Hypothermia
(physiopathology)
- Hypothermia, Induced
(methods)
- Hypoxia-Ischemia, Brain
(therapy)
- Inflammation
- Microglia
(metabolism)
- Myelin Sheath
(metabolism)
- Oligodendroglia
(pathology)
- Sheep
- Time Factors
- White Matter
(pathology)
|