Novel cuminaldehyde self-emulsified nanoemulsion for enhanced antihepatotoxicity in carbon tetrachloride-treated mice.

Abstract

OBJECTIVES:

Cuminaldehyde self-emulsified nanoemulsion (CuA-SEN) was prepared and optimised to improve its oral bioavailability and antihepatotoxicity.

METHODS:

Cuminaldehyde self-emulsified nanoemulsion was developed through the self-nanoemulsification method using Box-Behnken Design (BBD) tool while appropriate physicochemical indices were evaluated. The optimised CuA-SEN was characterised via droplet size (DS), morphology, polydispersity index (PDI), zeta potential (ZP), entrapment efficiency, in-vitro release, and pharmacokinetic studies while its antihepatotoxicity was evaluated.

KEY FINDINGS:

Cuminaldehyde self-emulsified nanoemulsion with acceptable characteristics (mean DS-48.83 ± 1.06 nm; PDI-0.232 ± 0.140; ZP-29.92 ± 1.66 mV; EE-91.51 ± 0.44%; and drug-loading capacity (DL)-9.77 ± 0.75%) was formulated. In-vitro drug release of CuA-SEN significantly increased with an oral relative bioavailability of 171.02%. Oral administration of CuA-SEN to CCl4 -induced hepatotoxicity mice markedly increased the levels of superoxide dismutase, glutathione and catalase in serum. Also, CuA-SEN reduced the levels of tumour necrosis factor-alpha and interleukin-6 in both serum and liver tissues while aspartate aminotransferase, alanine aminotransferase and malonaldehyde levels were significantly decreased.

CONCLUSIONS:

These findings showed that the improved bioavailability of cuminaldehyde via SEN provided an effective approach for enhancing antioxidation, anti-inflammation and antihepatotoxicity of the drug.

• Authors: Michael Adu-Frimpong, Wei Qiuyu, Caleb Kesse Firempong, Yusif Mohammed Mukhtar, Qiuxuan Yang, Emmanuel Omari-Siaw, Zhen Lijun, Ximing Xu, Jiangnan Yu
• Journal: The Journal of pharmacy and pharmacology (J Pharm Pharmacol) Vol. 71 Issue 8 Pg. 1324-1338 (Aug 2019) ISSN: 2042-7158 [Electronic] England
• PMID: 31168820 (Publication Type: Journal Article)
• Copyright: © 2019 Royal Pharmaceutical Society.

Chemical References

• Benzaldehydes
• Cymenes
• Emulsions
• Carbon Tetrachloride
• Catalase
• Superoxide Dismutase
• Glutathione
• cuminaldehyde

Topics

• Animals
• Benzaldehydes (blood, pharmacokinetics, pharmacology)
• Biological Availability
• Carbon Tetrachloride (adverse effects)
• Catalase (blood)
• Chemical and Drug Induced Liver Injury (drug therapy)
• Cymenes (blood, pharmacokinetics, pharmacology)
• Drug Liberation (drug effects)
• Emulsions (pharmacokinetics, pharmacology)
• Glutathione (blood)
• Male
• Mice
• Nanostructures (administration & dosage)
• Particle Size
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (blood)