The human nephrin Y1139RSL motif is essential for podocyte foot process organization and slit diaphragm formation during glomerular development.

Abstract

Nephrin is an immunoglobulin-type cell-adhesion molecule with a key role in the glomerular interpodocyte slit diaphragm. Mutations in the nephrin gene are associated with defects in the slit diaphragm, leading to early-onset nephrotic syndrome, typically resistant to treatment. Although the endocytic trafficking of nephrin is essential for the assembly of the slit diaphragm, nephrin's specific endocytic motifs remain unknown. To search for endocytic motifs, here we performed a multisequence alignment of nephrin and identified a canonical YXXØ-type motif, Y1139RSL, in the nephrin cytoplasmic tail, expressed only in primates. Using site-directed mutagenesis, various biochemical methods, single-plane illumination microscopy, a human podocyte line, and a human nephrin-expressing zebrafish model, we found that Y1139RSL is a novel endocytic motif and a structural element for clathrin-mediated nephrin endocytosis that functions as a phosphorylation-sensitive signal. We observed that Y1139RSL motif-mediated endocytosis helps to localize nephrin to specialized plasma membrane domains in podocytes and is essential for normal foot process organization into a functional slit diaphragm between neighboring foot processes in zebrafish. The importance of nephrin Y1139RSL for healthy podocyte development was supported by population-level analyses of genetic variations at this motif, revealing that such variations are very rare, suggesting that mutations in this motif have autosomal-recessive negative effects on kidney health. These findings expand our understanding of the mechanism underlying nephrin endocytosis and may lead to improved diagnostic tools or therapeutic strategies for managing early-onset, treatment-resistant nephrotic syndrome.

• Authors: Eugenel B Espiritu, Huajun Jiang, Sophie Moreau-Marquis, Mara Sullivan, Kunimasa Yan, Donna Beer Stolz, Matthew G Sampson, Neil A Hukriede, Agnieszka Swiatecka-Urban
• Journal: The Journal of biological chemistry (J Biol Chem) Vol. 294 Issue 28 Pg. 10773-10788 (07 12 2019) ISSN: 1083-351X [Electronic] United States
• PMID: 31152064 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: © 2019 Espiritu et al.

Chemical References

• Clathrin
• Membrane Proteins
• Morpholinos
• nephrin

Topics

• Amino Acid Motifs
• Animals
• Cell Line
• Cell Membrane (metabolism)
• Cell Movement
• Clathrin (metabolism)
• Embryo, Nonmammalian (metabolism)
• Endocytosis
• Humans
• Kidney Glomerulus (metabolism, ultrastructure)
• Membrane Proteins (antagonists & inhibitors, genetics, metabolism)
• Morpholinos (metabolism)
• Mutagenesis, Site-Directed
• Phosphorylation
• Podocytes (cytology, metabolism)
• Zebrafish (growth & development)