The quantitative determination of
sarcosine is of great importance in clinical chemistry, food and fermentation industries. Elevated
sarcosine levels are associated with
Alzheimer, dementia,
prostate cancer,
colorectal cancer,
stomach cancer and
sarcosinemia. This review summarizes the various methods for quantitative analysis of
sarcosine with special emphasis on various strategies of biosensors and their analytical performance. The current bio sensing methods have overcome the drawbacks of conventional methods.
Sarcosine biosensors work optimally at pH 7.0 to 8.0 in the linear range of 0.1 to 100 μM within 2 to 17 s and between 25 and 37 °C, within a limit of detection (LOD) between 0.008 and 500 mM. The formulated biosensors can be reused within a stability period of 3-180 days. Future research could be focused to modify existing
sarcosine biosensors, leading to simple, reliable, and economical sensors ideally suited for point-of-care treatment. Clinical significance Elevated
sarcosine levels are associated with prostate and
colorectal cancer,
Alzheimer, dementia,
stomach cancer and
sarcosinemia. Quantitative determination of
sarcosine is of great importance in clinical chemistry as well as food and fermentation industries. Attempts made in development of
sarcosine biosensors have been reviewed with their advantages and disadvantages, so that scientist and clinicians can improvise the methods of developing more potent
sarcosine biosensor applicable in multitudinous fields. This is the first comprehensive review which compares the various immobilization methods, sensing principles, strategies used in biosensors and their analytical performance in detail.