Abstract | OBJECTIVES: METHODS: The effect of T3, thyronamines, and Tetrac on the expression of TAAR1 in breast cancer cell lines MCF-7 and T47D was analyzed via PCR and Western blot. A MTT assay was performed to test the metabolic cell viability. A scratch assay was performed to analyze cell migration. RESULTS: Stimulation of MCF-7 cells with 10 nM 3-iodothyronamine (T1AM) significantly increased TAAR1 protein expression (P=0.008). In T47D cells, TAAR1 expression was significantly upregulated after the addition of 10 µg/mL estradiol to 10 nM T1AM (P=0.008). A significant (P=0.028) reduction in MCF-7 cell viability through the incubation with T1AM could be detected. Cell migration of MCF cells was significantly reduced through incubation with 10 nM T1AM. CONCLUSION: A significant upregulation of TAAR1 induced by stimulation with T1AM may be a sign for an increased decarboxylation of thyroid hormones in breast cancer cells. In addition, there seems to be an influence of estradiol for the T1AM-induced upregulation of TAAR1 in T47D cells. TAAR1-related cell transduction mechanisms seem to be an interesting target for endocrine treatment options of breast cancer patients.
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Authors | Eileen Tremmel, Simone Hofmann, Christina Kuhn, Helene Heidegger, Sabine Heublein, Kerstin Hermelink, Rachel Wuerstlein, Nadia Harbeck, Doris Mayr, Sven Mahner, Nina Ditsch, Udo Jeschke, Aurelia Vattai |
Journal | Breast cancer (Dove Medical Press)
(Breast Cancer (Dove Med Press))
Vol. 11
Pg. 87-97
( 2019)
ISSN: 1179-1314 [Print] New Zealand |
PMID | 30858725
(Publication Type: Journal Article)
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