Determination of prostate cancer biomarker acid phosphatase at a copper phthalocyanine-modified screen printed gold transducer.

Abstract

In this work, a novel sensor based on immobilised copper phthalocyanine, 2,9,16,23-tetracarboxylic acid-polyacrylamide (Cu(II)TC Pc-PAA) was developed for determination of acid phosphatase (ACP) levels in nanomolar quantities. Detection was based on the measurement of enzymatically generated phosphate, with initial studies focused on phosphate detection at a Cu(II)TC Pc-PAA modified screen-printed gold transducer. The sensor was characterised in relation to operational performance (pH, response time, stability, linearity, and sensitivity) and common anionic interferents (nitrate, sulphate, chloride, and perchlorate). The functionalised surface also facilitated rapid detection of the enzyme bi-product 2-naphthol over the range 5-3000 μM. Quantitation of ACP was demonstrated, realising a linear response range of 0.5-20 nM and LOD of 0.5 nM, which is within the clinical range for this prostate cancer biomarker.

• Authors: Zina Fredj, Mounir Ben Ali, Mohammed Nooredeen Abbas, Eithne Dempsey
• Journal: Analytica chimica acta (Anal Chim Acta) Vol. 1057 Pg. 98-105 (May 30 2019) ISSN: 1873-4324 [Electronic] Netherlands
• PMID: 30832923 (Publication Type: Journal Article)
• Copyright: Copyright © 2019 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Indoles
• Organometallic Compounds
• Perchlorates
• copper phthalocyanine
• Gold
• Acid Phosphatase
• Perchloric Acid

Topics

• Acid Phosphatase (analysis)
• Biomarkers, Tumor (analysis)
• Electrochemistry
• Electrodes
• Gold (chemistry)
• Humans
• Hydrogen-Ion Concentration
• Indoles (chemistry)
• Limit of Detection
• Male
• Organometallic Compounds (chemistry)
• Perchlorates (chemistry)
• Printing
• Prostatic Neoplasms (metabolism)
• Surface Properties
• Time Factors
• Transducers