Abstract |
The proteins of sheep pox, goat pox, sheep and goat pox and lumpy skin disease (Neethling) viruses were labelled with [35S] methionine. The major structural polypeptides of these viruses co-migrated on polyacrylamide gels, demonstrating the very close biochemical relationship between them. Using the agar gel immunodiffusion (AGID) test with radiolabelled antigen preparations, a major common precipitating antigen was identified. This co-migrated on polyacrylamide gels with one of the major structural polypeptides [mol. wt. 67000 (67K)]. The use of [35S] methionine-labelled antigen preparations considerably improved the sensitivity of the AGID test as a diagnostic test for capripoxvirus antibody detection.
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Authors | R P Kitching, J M Hammond, D N Black |
Journal | The Journal of general virology
(J Gen Virol)
Vol. 67 ( Pt 1)
Pg. 139-48
(Jan 1986)
ISSN: 0022-1317 [Print] England |
PMID | 3080547
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Viral
- Sulfur Radioisotopes
- Viral Proteins
- Methionine
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Topics |
- Animals
- Antigens, Viral
(analysis)
- Cells, Cultured
- Goats
- Immunodiffusion
- Lumpy skin disease virus
(analysis)
- Male
- Methionine
(metabolism)
- Poxviridae
(analysis, immunology, isolation & purification)
- Sheep
- Species Specificity
- Sulfur Radioisotopes
- Testis
- Viral Proteins
(analysis)
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