In vitro induction of tetraploidy in mulberry (Morus alba L.).

Abstract

A high frequency of tetraploidy was induced in mulberry (Morus alba L.) through apical bud treatment under in vitro conditions. Apical buds from in vitro-grown plants were treated with three different concentrations (0.05, 0.1 and 0.2%) of colchicine in MS medium for 24 h. Tetraploidy at a frequency of 39.4±4.8% was obtained using 0.1% colchicine, whereas the frequency of tetraploidy was significantly reduced to 16.7±2.3% when 0.2% colchicine was used. Morphological, histological and cytological evidence indicated a phenotypic and genomic similarity of in vitro- with ex vitro-induced tetraploids. Rooting of tetraploids was on basal medium containing 2.6 μM NAA. The recovery of tetraploids was 80.8% more efficient using the in vitro method instead of the ex vitro method. The use of the same colchicine medium for up to 4 weeks with additional explants was found to be equally effective for the induction of tetraploidy.

• Authors: S P Chakraborti, K Vijayan, B N Roy, S M H Qadri
• Journal: Plant cell reports (Plant Cell Rep) Vol. 17 Issue 10 Pg. 799-803 (Jul 1998) ISSN: 1432-203X [Electronic] Germany
• PMID: 30736595 (Publication Type: Journal Article)