Abstract | BACKGROUND: High-grade B-cell lymphomas (HGBCL) with MYC and BCL2 or/and BCL6 rearrangements (R), so-called double/triple-hit lymphomas (DH/THL), are uncommon, clinically aggressive lymphomas that require a prompt diagnosis. We aim to identify flow cytometric immunophenotypic (IP) features of DH/THL that may aid in triaging these cases followed by a timely confirmatory cytogenetic study. METHODS: RESULTS: Bright CD38 expression (CD38bright ) analyzed either qualitatively or semi-quantitatively was more common in DH/THL (56%) than in MYCneg DLBCL (17%) but less common compared to SHL (82%), indicating that CD38bright can serve as a biomarker for DH/THL. Additionally, CD38bright may be a better indicator for predicting DH/THL-BCL2 than DHL-BCL6, and very bright CD38 expression was exclusive to MYC rearranged lymphomas. The expression patterns of other markers were similar among these lymphoma groups. CONCLUSIONS: CD38bright is a biomarker associated with DH/THL with a moderate sensitivity (~50%) and high specificity (~90%). While this marker cannot be used as a screening tool, awareness of this correlation may aid in expediting the diagnosis and prioritizing FISH testing in resource limited settings or situations when samples are limited. Future studies to combine immunohistochemical markers are needed to further enhance the predictive power of CD38bright in diagnosing DH/THL. © 2019 International Clinical Cytometry Society.
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Authors | Abdullah Alsuwaidan, Elaina Pirruccello, Jesse Jaso, Prasad Koduru, Rolando Garcia, JoEllen Krueger, Meggie Doucet, Rahman Chaudhry, Franklin Fuda, Weina Chen |
Journal | Cytometry. Part B, Clinical cytometry
(Cytometry B Clin Cytom)
Vol. 96
Issue 5
Pg. 368-374
(09 2019)
ISSN: 1552-4957 [Electronic] United States |
PMID | 30734478
(Publication Type: Journal Article)
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Copyright | © 2019 International Clinical Cytometry Society. |
Chemical References |
- Biomarkers, Tumor
- Membrane Glycoproteins
- CD38 protein, human
- ADP-ribosyl Cyclase 1
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Topics |
- ADP-ribosyl Cyclase 1
(analysis, genetics)
- Biomarkers, Tumor
(analysis, genetics)
- Cohort Studies
- Female
- Flow Cytometry
- Humans
- Immunophenotyping
- Lymphoma, B-Cell
(diagnosis)
- Male
- Membrane Glycoproteins
(analysis, genetics)
- Sensitivity and Specificity
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