Effects of Microbial Metabolites of (-)-Epigallocatechin Gallate on Glucose Uptake in L6 Skeletal Muscle Cell and Glucose Tolerance in ICR Mice.

Abstract

Glucose uptake ability into L6 skeletal muscle cell was examined with eleven kinds of ring fission metabolites of (-)-epigallocatechin gallate (EGCG) produced by intestinal bacteria. The metabolites 5-(3,5-dihydroxyphenyl)-γ-valerolactone (EGC-M5), 4-hydroxy-5-(3,4,5-trihydroxyphenyl)valeric acid (EGC-M6), 5-(3,4,5-trihydroxyphenyl)-γ-valerolactone (EGC-M7) and 5-(3-hydroxyphenyl)valeric acid (EGC-M11) have been found to promote uptake of glucose into L6 myotubes significantly. EGC-M5, which is one of the major ring fission metabolites of EGCG, was also found to have a promotive effect on glucose transporter 4 (GLUT4) translocation accompanied by phosphorylation of AMP-activated protein kinase (AMPK) signaling pathway in skeletal muscle both in vivo and in vitro. Furthermore, the effect of oral single dosage of EGC-M5 on glucose tolerance test with ICR mice was examined and significant suppression of hyperglycemia was observed. These data suggested that EGC-M5 has an antidiabetic effect in vivo.

• Authors: Akiko Takagaki, Yasukiyo Yoshioka, Yoko Yamashita, Tomoya Nagano, Masaki Ikeda, Aya Hara-Terawaki, Ryota Seto, Hitoshi Ashida
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 42 Issue 2 Pg. 212-221 ( 2019) ISSN: 1347-5215 [Electronic] Japan
• PMID: 30713253 (Publication Type: Journal Article)

Chemical References

• 5-(3,5-dihydroxyphenyl)-gamma-valerolactone
• Blood Glucose
• Hypoglycemic Agents
• Lactones
• Catechin
• epigallocatechin gallate
• AMP-Activated Protein Kinases
• Glucose
• gamma-valerolactone

Topics

• AMP-Activated Protein Kinases (metabolism)
• Animals
• Blood Glucose (metabolism)
• Catechin (analogs & derivatives, chemistry, metabolism, pharmacology)
• Cell Line
• Gastrointestinal Microbiome
• Glucose (metabolism)
• Glucose Tolerance Test
• Hypoglycemic Agents
• Lactones (metabolism, pharmacology)
• Male
• Mice
• Mice, Inbred ICR
• Muscle Fibers, Skeletal (drug effects, metabolism)
• Myoblasts, Skeletal (drug effects, metabolism)
• Phosphorylation
• Signal Transduction (drug effects)