A Microbiota-Derived Bacteriocin Targets the Host to Confer Diarrhea Resistance in Early-Weaned Piglets.
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| Abstract |
Alternatives to antibiotics for preventing diarrhea in early-weaned farm animals are sorely needed. CM piglets (a native Chinese breed) are more resistant to early-weaning stress-induced diarrhea than the commercial crossbred LY piglets. Transferring fecal microbiota, but not saline, from healthy CM into LY piglets by oral administration prior to early weaning conferred diarrhea resistance. By comparing the relative abundance of intestinal microbiota in saline and microbiota transferred LY piglets, we identified and validated Lactobacillus gasseri LA39 and Lactobacillus frumenti as two bacterial species that mediate diarrhea resistance. Diarrhea resistance depended on the bacterial secretory circular peptide gassericin A, a bacteriocin. The binding of gassericin A to Keratin 19 (KRT19) on the plasma membrane of intestinal epithelial cells was essential for enhancement of fluid absorption and decreased secretion. These findings suggest the use of L. gasseri LA39 and L. frumenti as antibiotic alternatives for preventing diarrhea in mammals.
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| Authors | Jun Hu, Libao Ma, Yangfan Nie, Jianwei Chen, Wenyong Zheng, Xinkai Wang, Chunlin Xie, Zilong Zheng, Zhichang Wang, Tao Yang, Min Shi, Lingli Chen, Qiliang Hou, Yaorong Niu, Xiaofan Xu, Yuhua Zhu, Yong Zhang, Hong Wei, Xianghua Yan |
| Journal | Cell host & microbe (Cell Host Microbe) Vol. 24 Issue 6 Pg. 817-832.e8 (12 12 2018) ISSN: 1934-6069 [Electronic] United States |
| PMID | 30543777 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
| Copyright | Copyright © 2018 Elsevier Inc. All rights reserved. |
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