Abstract | PURPOSE: MATERIAL AND METHODS: We included in this study a total of 138 subjects with non-invasive diagnosis of non-alcoholic hepatic steatosis. The patatin-like phospholipase domain containing protein 3 (PNPLA3) rs738409 (adiponutrine) polymorphism was genotyped by allelic discrimination TaqMan PCR assay (5' nuclease assay), using predesigned TaqMan SNP Genotyping Assays. Class I and II HLA antigens were determined by the polymerase chain reaction sequence specific oligonucleotide method (ADN-PCR-SSO). The results were compared with the same data from the control group subjects. RESULTS: For PNPLA 3 polymorphism we found [CC] genotype in 82 subjects (59,42%), [GC] genotype in 45 (32,61%) and [GG] genotype in 11 subjects (7,97%). The frequency of minor [G] risk allele was 0.25. We found class I and II HLA antigens HLA A24, HLA B15, HLA DR15, HLA DR16, HLA DQ3 and HLA DQ5 more frequent in subjects with hepatic steatosis without any other risk factor and HLA-A2, HLA-32, HLA B18, HLA B49 and HLA B53 in patients with obesity or metabolic syndrome. CONCLUSIONS:
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Authors | A M Amzolini, M Fortofoiu, S E Tudorica-Micu, M C Fortofoiu, D Neagoe, M Popescu, F Burada, C C Vere, T Ciurea |
Journal | Current health sciences journal
(Curr Health Sci J)
2015 Oct-Dec
Vol. 41
Issue 4
Pg. 297-301
ISSN: 2067-0656 [Print] Romania |
PMID | 30538833
(Publication Type: Journal Article)
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