Actin related protein 3 (ARP3) promotes apoptosis of intestinal epithelial cells in ulcerative colitis.

Abstract

Apoptosis in intestinal epithelial cells (IECs) promotes the development of ulcerative colitis (UC), a type of inflammatory bowel disease (IBD). Efficient clearance of apoptotic cells is essential for tissue homeostasis in metazoans. Actin related protein 3 (ARP3) promotes endothelial dysfunction. The expression and function of ARP3 in UC remains unclear. In this study, the expression of apoptotic markers as p53, Bax, Cleaved-Caspease9 and Cleaved-Caspease3 were proved to be increased in the intestinal epithelial cells (IECs) of UC patients and in a mouse disuccinimidyl suberate(DSS)-induced colitis model; meanwhile, ARP3 expression was elevated. ARP3 expression levels and the severity of symptoms in patients with UC were positively correlated. By knocking down ARP3 in a TNF-α-treated NCM-460 cell colitis model, the apoptotic markers described above were all decreased. In conclusion, our data indicates that ARP3 might promote the apoptosis of IECs in UC, revealing a potential molecular target for treating UC.

• Authors: Shi-Qing Zhang, Wen-Kai Ni, Ming-Bing Xiao, Feng Jiang, Cui-Hua Lu, Rui-Hua Wang, Run-Zhou Ni
• Journal: Pathology, research and practice (Pathol Res Pract) Vol. 215 Issue 2 Pg. 235-242 (Feb 2019) ISSN: 1618-0631 [Electronic] Germany
• PMID: 30396755 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 Elsevier GmbH. All rights reserved.

Chemical References

• Actin-Related Protein 3

Topics

• Actin-Related Protein 3 (metabolism)
• Animals
• Apoptosis (physiology)
• Cell Line
• Colitis, Ulcerative (metabolism, pathology)
• Epithelial Cells (metabolism, pathology)
• Humans
• Intestinal Mucosa (metabolism, pathology)
• Mice
• Mice, Inbred C57BL