Abstract |
Ambient ionization mass spectrometric methods including desorption electrospray ionization (DESI) and atmospheric solid analysis probe (ASAP) have great potential for applications requiring real-time screening of target molecules in complex matrixes. Such techniques can also rapidly produce repeatable semiquantitative data, with minimal sample preparation, relative to liquid chromatography-mass spectrometry (LC-MS). In this study, a commercial ASAP probe was used to conduct both ASAP-MS and modified DESI (MDESI) MS analyses. We conducted real-time qualitative and semiquantitative analysis of the leanness-enhancing agent zilpaterol in incurred sheep urine, kidney, muscle, liver, and lung samples using ASAP-MS and MDESI MS. Using ASAP, limits of detection (LOD) and quantitation (LOQ) in urine were 1.1 and 3.7 ng/mL, respectively, while for MDESI MS they were 1.3 and 4.4 ng/mL, respectively. The LODs for tissues were 0.1-0.4 ng/g using ASAP, and 0.2-0.6 ng/g with MDESI MS. The LOQs of the tissues in ASAP were 0.4-1.2 ng/g and 0.5-2.1 ng/g in MDESI MS. Trace levels of zilpaterol were accurately analyzed in urine and tissues of sheep treated with dietary zilpaterol HCl. The correlation coefficient ( R2) between semiquantitative ASAP-MS and MDESI MS results of urine samples was 0.872. The data from ASAP and MDESI MS were validated using LC-MS/MS; urinary zilpaterol concentrations ≥5.0 ng/mL or tissue zilpaterol concentrations ≥1.5 ng/g were detected by ASAP and MDESI MS, respectively, 100% of the time. Forty samples could be analyzed in triplicate, directly from biological matrixes in under an hour.
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Authors | Shubhashis Chakrabarty, Weilin L Shelver, Heldur Hakk, David J Smith |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 66
Issue 41
Pg. 10871-10880
(Oct 17 2018)
ISSN: 1520-5118 [Electronic] United States |
PMID | 30295475
(Publication Type: Journal Article)
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Chemical References |
- Trimethylsilyl Compounds
- Zilpaterol
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Topics |
- Animals
- Body Fluids
(metabolism)
- Chromatography, High Pressure Liquid
(methods)
- Drug Residues
(analysis)
- High-Throughput Screening Assays
(methods)
- Humans
- Limit of Detection
- Sheep
(metabolism)
- Spectrometry, Mass, Electrospray Ionization
(methods)
- Tandem Mass Spectrometry
(methods)
- Trimethylsilyl Compounds
(metabolism, urine)
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