A new acute transforming feline retrovirus with fms homology specifies a C-terminally truncated version of the c-fms protein that is different from SM-feline sarcoma virus v-fms protein.

Abstract

The HZ5-feline sarcoma virus (FeSV) is a new acute transforming feline retrovirus which was isolated from a multicentric fibrosarcoma of a domestic cat. The HZ5-FeSV transforms fibroblasts in vitro and is replication defective. A biologically active integrated HZ5-FeSV provirus was molecularly cloned from cellular DNA of HZ5-FeSV-infected FRE-3A rat cells. The HZ5-FeSV has oncogene homology with the fms sequences of the SM-FeSV. The genome organization of the 8.6-kilobase HZ5-FeSV provirus is 5' delta gag-fms-delta pol-delta env 3'. The HZ5-and SM-FeSVs display indistinguishable in vitro transformation characteristics, and the structures of the gag-fms transforming genes in the two viruses are very similar. In the HZ5-FeSV and the SM-FeSV, identical c-fms and feline leukemia virus p10 sequences form the 5' gag-fms junction. With regard to v-fms the two viruses are homologous up to 11 amino acids before the C terminus of the SM-FeSV v-fms protein. In HZ5-FeSV a segment of 362 nucleotides then follows before the 3' recombination site with feline leukemia virus pol. The new 3' v-fms sequence encodes 27 amino acids before reaching a TGA termination signal. The relationship of this sequence with the recently characterized human c-fms sequence has been examined. The 3' HZ5-FeSV v-fms sequence is homologous with 3' c-fms sequences. A frameshift mutation (11-base-pair deletion) was found in the C-terminal fms coding sequence of the HZ5-FeSV. As a result, the HZ5-FeSV v-fms protein is predicted to be a C-terminally truncated version of c-fms. This frameshift mutation may determine the oncogenic properties of v-fms in the HZ5-FeSV.

• Authors: P Besmer, E Lader, P C George, P J Bergold, F H Qiu, E E Zuckerman, W D Hardy
• Journal: Journal of virology (J Virol) Vol. 60 Issue 1 Pg. 194-203 (Oct 1986) ISSN: 0022-538X [Print] United States
• PMID: 3018286 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Oncogene Proteins, Viral
• Proto-Oncogene Proteins

Topics

• Animals
• Base Sequence
• Cat Diseases (microbiology)
• Cats
• Cell Transformation, Viral
• Fibrosarcoma (microbiology, veterinary)
• Genes, Viral
• Oncogene Proteins, Viral (genetics)
• Oncogenes
• Proto-Oncogene Proteins (genetics)
• Recombination, Genetic
• Retroviridae (genetics)
• Sarcoma Viruses, Feline (genetics, isolation & purification)
• Sequence Homology, Nucleic Acid