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A sensitive fluorometric DNA nanobiosensor based on a new fluorophore for tumor suppressor gene detection.

Abstract
In this study, a sensitive fluorescent DNA nanobiosensor has been developed to determine DNA sequence of a well-known tumor suppressor gene, Adenomatous Polyposis Coli (APC). The design of the nanobiosensor was carried out using a synthetic organic ligand as a new fluorophore. The response mechanism of the nanobiosensor was based on DNA hybridization. The new fluorophore was assembled on gold nanoparticles (Au NPs) to enhance the sensitivity of the nanobiosensor response. The fabricated DNA nanobiosensor showed a fluorescence emission at 477 nm by exciting wavelength of 360 nm. By addition of the ssDNA target, the fluorescent emission of the nanobiosensor enhanced linearly in the range from 3.3 × 10-10 to 1.1 × 10-9 mol L-1 with detection limit of 1.3 × 10-11 mol L-1. The proposed DNA nanobiosensor responded selectively to its complementary strand in comparison with non-complementary and three mismatched bases. The nanobiosensor had also a fast response time with acceptable repeatability. Finally, the performance of the DNA nanobiosensor in biological fluid, serum plasma, was investigated and a satisfactory results were obtained.
AuthorsMaryam Darestani-Farahani, Farnoush Faridbod, Mohammad Reza Ganjali
JournalTalanta (Talanta) Vol. 190 Pg. 140-146 (Dec 01 2018) ISSN: 1873-3573 [Electronic] Netherlands
PMID30172490 (Publication Type: Journal Article)
CopyrightCopyright © 2018 Elsevier B.V. All rights reserved.
Chemical References
  • APC protein, human
  • Adenomatous Polyposis Coli Protein
  • DNA, Single-Stranded
  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Gold
Topics
  • Adenomatous Polyposis Coli Protein (blood, chemistry, genetics)
  • Base Sequence
  • Biosensing Techniques (methods)
  • DNA, Single-Stranded (chemistry, genetics)
  • Fluorescent Dyes (chemistry)
  • Fluorometry
  • Gold (chemistry)
  • Humans
  • Limit of Detection
  • Metal Nanoparticles (chemistry)
  • Nanotechnology (methods)
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes (chemistry, genetics)

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