Abietic acid attenuates IL-1β-induced inflammation in human osteoarthritis chondrocytes.

Abstract

Abietic acid has been reported to have anti-inflammatory activity. However, whether abietic acid has anti-inflammatory effects against osteoarthritis (OA) remains unclear. The present study aimed to measure the anti-inflammatory effects of abietic acid on OA in vitro. Human osteoarthritis chondrocytes were pretreated with abietic acid 1 h before IL-1β treatment. The results showed that treatment of abietic acid significantly inhibited IL-1β-induced TNF-α, NO, PGE2 production, and COX-2 expression. Abietic acid also concentration-dependently suppressed MMP1, MMP3, and MMP13 production induced by IL-1β. Moreover, the increased phosphorylation levels of NF-κB p65 and IκBα were inhibited by the treatment of abietic acid. We also found that the expression of PPAR-γ was increased by abietic acid. The inhibition of abietic acid on TNF-α, NO, and PGE2 production were reversed by GW9662, the inhibitor of PPAR-γ. In conclusion, the study elucidated abietic acid suppressed IL-1β-induced inflammation in human osteoarthritis chondrocytes by activating PPAR-γ.

• Authors: Sining Kang, Jun Zhang, Ye Yuan
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 64 Pg. 110-115 (Nov 2018) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 30172103 (Publication Type: Journal Article)
• Copyright: Copyright © 2018. Published by Elsevier B.V.

Chemical References

• Abietanes
• Anti-Inflammatory Agents
• Inflammation Mediators
• Interleukin-1beta
• NF-kappa B
• PPAR gamma
• Metalloproteases
• Dinoprostone
• abietic acid

Topics

• Abietanes (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Cell Survival (drug effects)
• Cells, Cultured
• Chondrocytes (drug effects, physiology)
• Dinoprostone (biosynthesis)
• Humans
• Inflammation Mediators (metabolism)
• Interleukin-1beta (antagonists & inhibitors)
• Metalloproteases (biosynthesis)
• NF-kappa B (physiology)
• Osteoarthritis (drug therapy)
• PPAR gamma (physiology)