Noonan Syndrome-Associated SHP2 Dephosphorylates GluN2B to Regulate NMDA Receptor Function.

Abstract

Hyperactivating mutations in the non-receptor tyrosine phosphatase SHP2 cause Noonan syndrome (NS). NS is associated with cognitive deficits, but how hyperactivation of SHP2 in NS changes neuron function is not well understood. We find that mice bearing an NS-associated SHP2 allele (NS mice) have selectively impaired Schaffer collateral-CA1 NMDA (N-methyl-D-aspartate) receptor (NMDAR)-mediated neurotransmission and that residual NMDAR-mediated currents decay faster in NS mice because of reduced contribution of GluN1:GluN2B diheteromers. Consistent with altered GluN2B function, we identify GluN2B Y1252 as an NS-associated SHP2 substrate both in vitro and in vivo. Mutation of Y1252 does not alter recombinant GluN1:GluN2B receptor kinetics. Instead, phospho-Y1252 binds the actin-regulatory adaptor protein Nck2, and this interaction is required for proper NMDAR function. These results establish SHP2 and Nck2 as NMDAR regulatory proteins and strongly suggest that NMDAR dysfunction contributes to NS cognitive deficits.

• Authors: Aaron D Levy, Xiao Xiao, Juliana E Shaw, Suma Priya Sudarsana Devi, Sara Marie Katrancha, Anton M Bennett, Charles A Greer, James R Howe, Kazuya Machida, Anthony J Koleske
• Journal: Cell reports (Cell Rep) Vol. 24 Issue 6 Pg. 1523-1535 (08 07 2018) ISSN: 2211-1247 [Electronic] United States
• PMID: 30089263 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.

Chemical References

• NR2B NMDA receptor
• Receptors, N-Methyl-D-Aspartate
• Protein Tyrosine Phosphatase, Non-Receptor Type 11

Topics

• Animals
• Disease Models, Animal
• Humans
• Mice
• Noonan Syndrome (genetics, metabolism)
• Protein Tyrosine Phosphatase, Non-Receptor Type 11 (metabolism)
• Receptors, N-Methyl-D-Aspartate (metabolism)
• Signal Transduction