Protective effects of hyperbaric oxygen preconditioning against LPS-induced acute lung injury in rats.

Abstract

INTRODUCTION:

Acute lung injury (ALI) is generally caused by oxidative damages and pulmonary overinflammations. Hyperbaric oxygen preconditioning (HBO2-PC) has been proven protective against oxidative-stress-related injuries. In this study, we investigated the effect of HBO2-PC on lipopolysaccharide (LPS)-induced ALI in rats.

METHODS:

Thirty-two Sprague-Dawley rats randomly assigned into Sham, HBO2-PC, ALI and HBO2-PC÷ALI groups (eight in each group) were sacrificed at 12 hours after the injection of LPS. The severity of ALI in rats was assessed in terms of histopathological changes in addition to wet/dry weight ratios. The levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-6 and IL-1β in serum and lung homogenates were measured by enzyme-linked immunosorbent and qRT-PCR assays. Activities by hydrogen peroxide (H2O2), malondialdehyde (MDA), myeloperoxidase (MPO) as well as superoxide dismutase (SOD) in rat lungs were tested for neutrophil infiltration. Meanwhile the oxidative stress molecular markers nuclear factor-kappa B(NF-κB) p65 and nuclear factor erythroid 2-related factor 2 (Nrf2), together with its downstream heme-oxygenase 1 (HO-1) and NAD(P)H: quinone oxidoreductase 1 (NQO1) were also quantified.

RESULTS:

HBO2-PC significantly alleviated LPS-induced ALI, lowered the lung injury scores, reduced lung water content, and reduced H2O2, MDA levels as well as MPO activity, while simultaneously improving the arterial partial oxygen pressure (PaO2) and SOD activity. Furthermore, HBO2-PC inhibited the nuclear translocation of NF-κB p65 while enhancing the nuclear translocation of Nrf2, thus upregulating HO-1 and NQO1.

CONCLUSIONS:

Our results suggest that HBO2-PC was potentially protective for LPS-induced ALI lungs in rats, with a presumed mechanism that suppressed NF-κB while activating Nrf2. We propose that HBO2-PC should be considered a potential therapeutic strategy against ALI in rats.

• Authors: Chongfeng Bai, Tao Li, Yi Chen, Tongyi Xu, Xiaohong Wang, Jinjian Yu, Xuan Liu, Qing Sun
• Journal: Undersea & hyperbaric medicine : journal of the Undersea and Hyperbaric Medical Society, Inc (Undersea Hyperb Med) 2018 May-Jun Vol. 45 Issue 3 Pg. 323-333 ISSN: 1066-2936 [Print] United States
• PMID: 30028919 (Publication Type: Journal Article)
• Copyright: Copyright© Undersea and Hyperbaric Medical Society.

Chemical References

• Il6 protein, rat
• Interleukin-1beta
• Interleukin-6
• Lipopolysaccharides
• NF-E2-Related Factor 2
• NF-kappa B
• Nfe2l2 protein, rat
• Transcription Factor RelA
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Hydrogen Peroxide
• Heme Oxygenase-1
• Superoxide Dismutase
• NAD(P)H Dehydrogenase (Quinone)
• NQO1 protein, rat

Topics

• Acute Lung Injury (blood, etiology, prevention & control)
• Animals
• Heme Oxygenase-1 (analysis)
• Hydrogen Peroxide (analysis)
• Hyperbaric Oxygenation
• Interleukin-1beta (analysis)
• Interleukin-6 (analysis)
• Lipopolysaccharides
• Male
• Malondialdehyde (analysis)
• NAD(P)H Dehydrogenase (Quinone) (analysis)
• NF-E2-Related Factor 2 (analysis)
• NF-kappa B (analysis)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Superoxide Dismutase (analysis)
• Transcription Factor RelA (analysis)
• Tumor Necrosis Factor-alpha (analysis)