Synergistic effects of ischemic preconditioning and immediate post-conditioning in the protection against ischemia/reperfusion injury in rabbit submandibular glands.

Abstract

Submandibular gland autotransplantation is an effective approach for treating severe keratoconjunctivitis sicca. However, ischemia/reperfusion (I/R) injury, which inevitably occurs during transplantation, is involved in the hypofunction and structural damage that occur early after transplantation. Therefore, it is critical to identify effective strategies to ameliorate I/R injury in submandibular glands. In this study, we investigated the ability of immediate post-conditioning combined with ischemic preconditioning to attenuate I/R injury. We observed that after I/R injury, the level of reactive oxygen species was increased, inflammatory response was strengthened, and severe apoptosis had occurred. In addition, the salivary flow rate was greatly decreased. However, the pathogenesis of I/R injury was significantly ameliorated by ischemia post-conditioning or ischemia preconditioning treatments. In addition, the combination of ischemia preconditioning and post-conditioning achieved synergistic protective effects against I/R injury compared with ischemia preconditioning or ischemia post-conditioning alone. The secretion function was restored in the combination group. Furthermore, the combination treatment involved the same mechanisms of ischemia preconditioning or ischemia post-conditioning, including suppression of the inflammatory reaction and neutrophil accumulation, attenuation of oxidation stress, and inhibition of apoptosis. In conclusion, the combination of ischemia preconditioning and ischemia post-conditioning treatment is a simple and effective approach for treating I/R injury in submandibular glands.

• Authors: Si-En Zhang, Shi-Hong Wen, Yu-Xiong Su, Guang-Sen Zheng, Di-Kan Wang, Yu-Jie Liang, Gui-Qing Liao
• Journal: European journal of oral sciences (Eur J Oral Sci) Vol. 126 Issue 4 Pg. 282-291 (08 2018) ISSN: 1600-0722 [Electronic] England
• PMID: 30006965 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2018 Eur J Oral Sci.

Chemical References

• Lactic Acid
• Malondialdehyde
• Peroxidase
• Reactive Oxygen Species
• Superoxide Dismutase

Topics

• Animals
• Male
• Rabbits
• Apoptosis
• Blotting, Western
• Enzyme-Linked Immunosorbent Assay
• In Situ Nick-End Labeling
• Ischemic Preconditioning
• Lactic Acid (metabolism)
• Malondialdehyde (metabolism)
• Peroxidase (metabolism)
• Random Allocation
• Reactive Oxygen Species (metabolism)
• Real-Time Polymerase Chain Reaction
• Reperfusion Injury (metabolism, physiopathology, prevention & control)
• Salivation
• Submandibular Gland (injuries, metabolism)
• Superoxide Dismutase (metabolism)