Knockdown of GA-binding protein subunit β1 inhibits cell proliferation via p21 induction in renal cell carcinoma.

Abstract	Clear cell renal cell carcinoma (ccRCC) is the most common type of renal cancer. In the present study, bioinformatics tools were systematically used to investigate the potential upstream effector involved in the progression of ccRCC. Using the Gene Expression Omnibus database and Library of Integrated Network-based Cellular Signatures L1000 platform, it was identified that GA-binding protein subunit β1 (GABPB1) was a potential effector gene. GABPB1 is a transcription factor subunit and its function in ccRCC is unclear. Elevated expression of GABPB1 mRNA in ccRCC was also observed in other clinical datasets from the Oncomine database. Following reverse transcription-quantitative polymerase chain reaction and western blot analysis, the ccRCC 786-O and A498 cell lines showed higher expression levels of GABPB1 than HK-2, a normal kidney cell line. Knockdown of GABPB1 in the 786-O and A498 cells significantly decreased the ability to form colonies by inducing the expression of p21Waf/Cip1. SurvExpress database analysis indicated that a higher expression of GABPB1 was associated with poor survival outcome in patients with renal cancer. These findings imply that GABPB1 serves an important role in the progression of ccRCC.
Authors	Szu-Chia Chen, Meng-Chi Yen, Feng-Wei Chen, Ling-Yu Wu, Shiang-Jie Yang, Po-Lin Kuo, Ya-Ling Hsu
Journal	International journal of oncology (Int J Oncol) Vol. 53 Issue 2 Pg. 886-894 (Aug 2018) ISSN: 1791-2423 [Electronic] Greece
PMID	29845229 (Publication Type: Journal Article)
Chemical References	CDKN1A protein, human Cyclin-Dependent Kinase Inhibitor p21 GA-Binding Protein Transcription Factor GABPB1 protein, human
Topics	Carcinoma, Renal Cell (genetics, metabolism) Cell Line, Tumor Cell Movement Cell Proliferation Cyclin-Dependent Kinase Inhibitor p21 (genetics, metabolism) Disease Progression GA-Binding Protein Transcription Factor (genetics, metabolism) Gene Expression Regulation, Neoplastic Gene Knockdown Techniques Humans Kidney Neoplasms (genetics, metabolism) Prognosis Survival Analysis Up-Regulation

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