Screening and analysis of key active constituents in Guanxinshutong capsule using mass spectrum and integrative network pharmacology.

Abstract

Guanxinshutong capsule (GXSTC) is an effective and safe traditional Chinese medicine used in the treatment of cardiovascular diseases (CVDs) for many years. However, the targets of this herbal formula and the underlying molecular mechanisms of action involved in the treatment of CVDs are still unclear. In the present study, we used a systems pharmacology approach to identify the active ingredients of GXSTC and their corresponding targets in the calcium signaling pathway with respect to the treatment of CVDs. This method integrated chromatographic techniques, prediction of absorption, distribution, metabolism, and excretion, analysis using Kyoto Encyclopedia of Genes and Genomes, network construction, and pharmacological experiments. 12 active compounds and 33 targets were found to have a role in the treatment of CVDs, and four main active ingredients, including protocatechuic acid, cryptotanshinone, eugenol, and borneol were selected to verify the effect of (GXSTC) on calcium signaling system in cardiomyocyte injury induced by hypoxia and reoxygenation. The results from the present study revealed the active components and targets of GXSTC in the treatment of CVDs, providing a new perspective to enhance the understanding of the role of the calcium signaling pathway in the therapeutic effect of GXSTC.

• Authors: Feng Liu, Xia DU, Pei-Rong Liu, Yu-Hong Sun, Yan-Min Zhang
• Journal: Chinese journal of natural medicines (Chin J Nat Med) Vol. 16 Issue 4 Pg. 302-312 (Apr 2018) ISSN: 1875-5364 [Electronic] China
• PMID: 29703330 (Publication Type: Journal Article)
• Copyright: Copyright © 2018 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Chemical References

• Camphanes
• Cardiotonic Agents
• Drugs, Chinese Herbal
• Hydroxybenzoates
• Phenanthrenes
• Receptor, PAR-1
• guanxin shutong
• protocatechuic acid
• Eugenol
• cryptotanshinone
• NOS3 protein, human
• Nitric Oxide Synthase Type III
• isoborneol

Topics

• Animals
• Animals, Newborn
• Camphanes (chemistry)
• Cardiotonic Agents (chemistry, pharmacology)
• Cells, Cultured
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Eugenol (chemistry)
• Gene Expression (drug effects)
• Hydroxybenzoates (chemistry)
• Mass Spectrometry
• Models, Biological
• Myocytes, Cardiac (drug effects)
• Nitric Oxide Synthase Type III (genetics)
• Phenanthrenes (chemistry)
• Rats
• Rats, Sprague-Dawley
• Receptor, PAR-1 (genetics)
• Systems Biology