Cryomilled zinc sulfide: A prophylactic for Staphylococcus aureus-infected wounds.

Abstract

Bacterial pathogens that colonize wounds form biofilms, which protect the bacteria from the effect of host immune response and antibiotics. This study examined the effectiveness of newly synthesized zinc sulfide in inhibiting biofilm development by Staphylococcus aureus ( S. aureus) strains. Zinc sulfide (ZnS) was anaerobically biosynthesized to produce CompA, which was further processed by cryomilling to maximize the antibacterial properties to produce CompB. The effect of the two compounds on the S. aureus strain AH133 was compared using zone of inhibition assay. The compounds were formulated in a polyethylene glycol cream. We compared the effect of the two compounds on biofilm development by AH133 and two methicillin-resistant S. aureus clinical isolates using the in vitro model of wound infection. Zone of inhibition assay revealed that CompB is more effective than CompA. At 15 mg/application, the formulated cream of either compound inhibited biofilm development by AH133, which was confirmed using confocal laser scanning microscopy. At 20 mg/application, CompB inhibited biofilm development by the two methicillin-resistant S. aureus clinical isolates. To further validate the effectiveness of CompB, mice were treated using the murine model of wound infection. Colony forming cell assay and in vivo live imaging results strongly suggested the inhibition of S. aureus growth.

• Authors: Phat L Tran, Jianqiang Li, Lisa Lungaro, Srikanthan Ramesh, Ilia N Ivanov, Ji-Won Moon, David E Graham, Abdul Hamood, James Wang, Alistair Pd Elfick, Iris V Rivero
• Journal: Journal of biomaterials applications (J Biomater Appl) Vol. 33 Issue 1 Pg. 82-93 (07 2018) ISSN: 1530-8022 [Electronic] England
• PMID: 29683016 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Anti-Bacterial Agents
• Biocompatible Materials
• Sulfides
• Zinc Compounds
• Polyethylene Glycols
• zinc sulfide

Topics

• Animals
• Anti-Bacterial Agents (chemistry, therapeutic use)
• Antibiotic Prophylaxis
• Biocompatible Materials (chemistry)
• Biofilms
• Cell Survival (drug effects)
• Female
• Methicillin-Resistant Staphylococcus aureus (drug effects)
• Mice
• Microbial Sensitivity Tests
• Particle Size
• Polyethylene Glycols (chemistry)
• RAW 264.7 Cells
• Staphylococcal Infections (prevention & control)
• Sulfides (chemistry, therapeutic use)
• Surface Properties
• Wound Infection (drug therapy)
• Zinc Compounds (chemistry, therapeutic use)