The effect of rutin on ovarian ischemia-reperfusion injury in a rat model.

Abstract

The effect of rutin on ovarian ischemia-reperfusion (I/R) injury was investigated in this experimental study. Eighteen Wistar albino female rats were divided into three groups as follows: I/R group (IRG; n = 6), 50 mg/kg rutin + I/R group (RG; n = 6), and a healthy control group scheduled for a sham operation (SG; n = 6). 2 h of ischemia and following 2 h of reperfusion were created in the IRG and RG by using a torsion model involving atraumatic vascular clips. Rutin, a flavonoid glycoside, was injected intraperitoneally at the dose of 50 mg/kg to RG group 1 h before reperfusion. Then, rats were euthanized and their ovaries were removed for biochemical and histopathological examination and also assessment of the gene expressions. IRG group had a significant increase in malondialdehyde (MDA) levels, in the expressions of tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β), and also in the activity of cyclooxygenase 2 (COX-2) unlike the significant decrease in total glutathione (tGSH) levels and the activity of COX-1 when compared to the SG group. However, rutin significantly decreased MDA levels, the expressions of TNF-α and IL-1β, and also the activity of COX-2 while it increased significantly tGSH levels and the activity of COX-1 in the RG group in comparison with the IRG group. Rutin ameliorated the I/R-induced ovarian injury in rats via its possible antioxidative and anti-inflammatory effects.

• Authors: Cenk Nayki, Umit Nayki, Ferda Keskin Cimen, Mehmet Kulhan, Omer Erkan Yapca, Nezahat Kurt, Aslı Bilgin Ozbek
• Journal: Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology (Gynecol Endocrinol) Vol. 34 Issue 9 Pg. 809-814 (Sep 2018) ISSN: 1473-0766 [Electronic] England
• PMID: 29564969 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Interleukin-1beta
• Tumor Necrosis Factor-alpha
• Malondialdehyde
• Rutin

Topics

• Animals
• Antioxidants (pharmacology, therapeutic use)
• Female
• Injections, Intraperitoneal
• Interleukin-1beta (metabolism)
• Malondialdehyde (metabolism)
• Ovarian Diseases (drug therapy, metabolism)
• Ovary (blood supply, drug effects, metabolism)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Reperfusion Injury (drug therapy, metabolism)
• Rutin (pharmacology, therapeutic use)
• Tumor Necrosis Factor-alpha (metabolism)