In the present study, we examined the effects of Dendropanax morbifera Léveille leaf extract (DML) on
D-galactose-induced morphological changes in microglia and
cytokines, including pro-inflammatory
cytokines (
interleukin [IL]-1β, IL-6, and
tumor necrosis factor [TNF]-α) and anti-inflammatory
cytokines (IL-4 and IL-10) in the hippocampus. Administration of DML to
D-galactose-treated mice significantly improved
D-galactose-induced reduction in escape latency, swimming speed, and spatial preference for the target quadrant. In addition, administration of DML to
D-galactose-treated mice significantly ameliorated the microglial activation and increases of IL-1β,
IL-6, and TNF-α levels in the hippocampus. Administration of
D-galactose significantly reduced
IL-4 levels in the hippocampus, while administration of DML to
D-galactose-treated mice significantly increased
IL-4 level. However, we did not observe any significant changes in
IL-10 levels in hippocampal homogenates. These results suggest that DML reduces
D-galactose-induced mouse senescence by reducing pro-inflammatory
cytokines such as IL-1β,
IL-6, and TNF-α, as well as increasing anti-inflammatory
cytokine IL-4.