Abstract |
The eryptotic and hemolytic effects of a phytosterol (PS) mixture (β- sitosterol, campesterol, stigmasterol) or β- cryptoxanthin (β-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 μM) were evaluated. β- Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca2+ influx. Co-incubation of both bioactive compounds protected against β-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca2+ influx, ROS overproduction and GSH depletion at 75 μM, and hemolysis at both tBOOH concentrations. β- Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by β- Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.
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Authors | Andrea Alvarez-Sala, Gabriel López-García, Alessandro Attanzio, Luisa Tesoriere, Antonio Cilla, Reyes Barberá, Amparo Alegría |
Journal | Journal of agricultural and food chemistry
(J Agric Food Chem)
Vol. 66
Issue 5
Pg. 1157-1166
(Feb 07 2018)
ISSN: 1520-5118 [Electronic] United States |
PMID | 29345907
(Publication Type: Journal Article)
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Chemical References |
- Beta-Cryptoxanthin
- Phytosterols
- Sitosterols
- campesterol
- gamma-sitosterol
- tert-Butylhydroperoxide
- Cholesterol
- Stigmasterol
- Glutathione
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Topics |
- Beta-Cryptoxanthin
(blood, pharmacology)
- Cells, Cultured
- Cholesterol
(analogs & derivatives, pharmacology)
- Eryptosis
(drug effects)
- Erythrocytes
(chemistry, drug effects)
- Glutathione
(blood)
- Hemolysis
(drug effects)
- Humans
- Oxidative Stress
(drug effects)
- Phytosterols
(pharmacology)
- Sitosterols
(pharmacology)
- Stigmasterol
(pharmacology)
- tert-Butylhydroperoxide
(pharmacology)
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