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Effects of Plant Sterols or β-Cryptoxanthin at Physiological Serum Concentrations on Suicidal Erythrocyte Death.

Abstract
The eryptotic and hemolytic effects of a phytosterol (PS) mixture (β-sitosterol, campesterol, stigmasterol) or β-cryptoxanthin (β-Cx) at physiological serum concentration and their effect against oxidative stress induced by tert-butylhydroperoxide (tBOOH) (75 and 300 μM) were evaluated. β-Cryptoxanthin produced an increase in eryptotic cells, cell volume, hemolysis, and glutathione depletion (GSH) without ROS overproduction and intracellular Ca2+ influx. Co-incubation of both bioactive compounds protected against β-Cx-induced eryptosis. Under tBOOH stress, PS prevented eryptosis, reducing Ca2+ influx, ROS overproduction and GSH depletion at 75 μM, and hemolysis at both tBOOH concentrations. β-Cryptoxanthin showed no cytoprotective effect. Co-incubation with both bioactive compounds completely prevented hemolysis and partially prevented eryptosis as well as GSH depletion induced by β-Cx plus tBOOH. Phytosterols at physiological serum concentrations help to prevent pro-eryptotic and hemolytic effects and are promising candidate compounds for ameliorating eryptosis-associated diseases.
AuthorsAndrea Alvarez-Sala, Gabriel López-García, Alessandro Attanzio, Luisa Tesoriere, Antonio Cilla, Reyes Barberá, Amparo Alegría
JournalJournal of agricultural and food chemistry (J Agric Food Chem) Vol. 66 Issue 5 Pg. 1157-1166 (Feb 07 2018) ISSN: 1520-5118 [Electronic] United States
PMID29345907 (Publication Type: Journal Article)
Chemical References
  • Beta-Cryptoxanthin
  • Phytosterols
  • Sitosterols
  • campesterol
  • gamma-sitosterol
  • tert-Butylhydroperoxide
  • Cholesterol
  • Stigmasterol
  • Glutathione
Topics
  • Beta-Cryptoxanthin (blood, pharmacology)
  • Cells, Cultured
  • Cholesterol (analogs & derivatives, pharmacology)
  • Eryptosis (drug effects)
  • Erythrocytes (chemistry, drug effects)
  • Glutathione (blood)
  • Hemolysis (drug effects)
  • Humans
  • Oxidative Stress (drug effects)
  • Phytosterols (pharmacology)
  • Sitosterols (pharmacology)
  • Stigmasterol (pharmacology)
  • tert-Butylhydroperoxide (pharmacology)

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