Abstract | BACKGROUND: Dysregulated DNA methylation in lymphocytes has been linked to autoimmune disorders. The aims of this study were to identify global DNA methylation patterns in patients with autoimmune thyroid diseases and to observe methylation changes after treatment for these conditions. METHODS: RESULTS: Hypomethylation and down-regulated DNMT1 expression in T and B lymphocytes were observed in the newly diagnosed GD patients. Neither the AIT patients nor the positive thyroid autoantibodies patients exhibited differences in their global DNA methylation status or DNMT mRNA levels compared with healthy controls. Antithyroid drugs restored global methylation and DNMT1 expression in both T and B lymphocytes, whereas radioiodine therapy affected only T cells. L-thyroxine replacement did not alter the methylation or DNMT expression levels in lymphocytes. The global methylation levels of B cells were negatively correlated with the serum thyroid peroxidase antibodies in patients with autoimmune thyroid diseases. CONCLUSIONS:
Hyperthyroid patients with newly diagnosed GD had global hypomethylation and lower DNMT1 expression in T and B lymphocytes. The results provide the first demonstration that antithyroid drugs or radioiodine treatment restore global DNA methylation and DNMT1 expression with concurrent relief of hyperthyroidism.
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Authors | Qingling Guo, Dan Wu, Huixin Yu, Jiandong Bao, Shiqiao Peng, Zhongyan Shan, Haixia Guan, Weiping Teng |
Journal | Thyroid : official journal of the American Thyroid Association
(Thyroid)
Vol. 28
Issue 3
Pg. 377-385
(03 2018)
ISSN: 1557-9077 [Electronic] United States |
PMID | 29336230
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Iodine Radioisotopes
- Triiodothyronine
- Methimazole
- Propylthiouracil
- DNA (Cytosine-5-)-Methyltransferase 1
- DNMT1 protein, human
- Thyroxine
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Topics |
- Adult
- B-Lymphocytes
(metabolism)
- Cross-Sectional Studies
- DNA (Cytosine-5-)-Methyltransferase 1
(genetics, metabolism)
- DNA Methylation
- Female
- Graves Disease
(drug therapy, metabolism, radiotherapy)
- Hashimoto Disease
(drug therapy, metabolism)
- Humans
- Hypothyroidism
(drug therapy, metabolism)
- Iodine Radioisotopes
(therapeutic use)
- Male
- Methimazole
(therapeutic use)
- Middle Aged
- Propylthiouracil
(therapeutic use)
- T-Lymphocytes
(metabolism)
- Thyroiditis, Autoimmune
(drug therapy, metabolism)
- Thyroxine
(blood, therapeutic use)
- Triiodothyronine
(blood)
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