Human T cell recognition of cloned HLA class I gene products expressed on DNA transfectants of mouse mastocytoma P815.

Abstract

Cloned genes for human major histocompatibility complex (MHC) class I antigens were introduced by DNA-mediated gene transfer into a high-efficiency transfection recipient (HTR) cell line previously derived from mouse mastocytoma P815. Cell surface expression of HLA-A3, AW24 and CW3 gene products on P815 transfectants was demonstrated by radioimmune assay and by flow cytometry. The human MHC class I gene products were apparently expressed on P815 transfectants in a form recognized by human cytolytic T lymphocytes (CTL). Human CTL generated in unidirectional mixed lymphocyte culture against AW24+ donor lymphocytes clearly lysed P815-HLA-AW24+ transfectant target cells, but not untransfected P815(HTR) controls. Moreover, P815-HLA transfectants could stimulate in vitro a significant alloreactive human CTL response. Lysis of P815-HLA transfectant target cells by human CTL was inhibited by a monoclonal antibody directed against human MHC class I gene products. These mouse cell transfectants may be useful for the study of human T cell responses.

• Authors: J L Maryanski, A Moretta, B Jordan, E De Plaen, A Van Pel, T Boon, J C Cerottini
• Journal: European journal of immunology (Eur J Immunol) Vol. 15 Issue 11 Pg. 1111-7 (Nov 1985) ISSN: 0014-2980 [Print] Germany
• PMID: 2933264 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• HLA Antigens
• DNA

Topics

• Animals
• Antibodies, Monoclonal (physiology)
• Binding, Competitive
• Cloning, Molecular
• Cytotoxicity, Immunologic
• DNA (metabolism)
• Genes, MHC Class II
• HLA Antigens (genetics, immunology)
• Lymphocyte Activation
• Lymphocyte Culture Test, Mixed
• Mast-Cell Sarcoma (genetics, immunology)
• Mice
• T-Lymphocytes, Cytotoxic (immunology, metabolism)
• Transfection