Abstract | BACKGROUND: METHODS: C57BL/6 mice were randomized to receive oral PN with parenteral saline, FO, SO, or a mixture of 50% FO and 50% SO for 19 days. Fatty acid analysis, histologic evaluation, Nonalcoholic Steatohepatitis Clinical Research Network (NSCRN) scores, and reverse-transcriptase polymerase chain reaction for key lipogenic genes were performed. RESULTS: The PN + saline group was the only group with EFAD, with a serum and hepatic triene/tetraene ratio of 0.53. NSCRN scores were highest in the PN + SO group (5.5; 95% confidence interval [CI], 4.9-6.1), followed by the PN + FO/SO (4.5; 95% CI, 3.5-5.5) group, with the lowest score in the PN + FO (2.0; 95% CI, 1.1-2.9) group. Acetyl CoA carboxylase α and acetyl CoA carboxylase β expression was lower in the PN + FO group than in the PN + FO/SO or PN + SO groups. CONCLUSIONS: Our data demonstrate that a mixed fat emulsion of 50% SO and 50% FO is inferior to 100% FO in reducing hepatosteatosis in this model. These data suggest that use of parenteral SO with parenteral FO, in a 1:1 ratio, may still contribute to liver injury, although it is less hepatotoxic than pure SO.
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Authors | Prathima Nandivada, Gillian L Fell, Amy H Pan, Vania Nose, Paul D Mitchell, Kathleen M Gura, Mark Puder |
Journal | JPEN. Journal of parenteral and enteral nutrition
(JPEN J Parenter Enteral Nutr)
Vol. 42
Issue 2
Pg. 403-411
(02 2018)
ISSN: 1941-2444 [Electronic] United States |
PMID | 29187040
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2017 American Society for Parenteral and Enteral Nutrition. |
Chemical References |
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Topics |
- Animals
- Disease Models, Animal
- Fish Oils
(administration & dosage, pharmacology)
- Liver Diseases
(complications)
- Male
- Mice
- Mice, Inbred C57BL
- Non-alcoholic Fatty Liver Disease
(complications, diet therapy)
- Parenteral Nutrition
(methods)
- Soybean Oil
(administration & dosage, pharmacology)
- Treatment Outcome
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