DNA polymerase 5 acetylation by Eso1 is essential for Schizosaccharomyces pombe viability.

Abstract	Eco1/Eso1 protein plays an important role in chromosome segregation, DNA repair and gene regulation. Eco1 mutation induces Roberts syndrome clinically and rDNA transcription disorders in vivo. In this study, we examined the role of Eso1 protein binding to polymerase 5 (Pol5) and the acetylation of Pol5 protein in the regulation of Schizosaccharomyces pombe (S. pombe) viability. Immunoprecipitation and mass spectrometry assays identified Eso1 protein binding to Cdc2, Pol5 and Cdc21, as well as other proteins. Pol5 protein specifically bound to Eso1 protein, but not to the Rad30 part or Rad30 part plus the additional zinc finger domain of Eco1 protein. Mass spectrometry data further identified several acetylation or trimethylation modification sites in the lysine residues of the Pol5 protein. However, the mutation of the Pol5 K47 site to arginine was lethal to S. pombe. Eso1 protein was able to acetylate Pol5 protein and mediate S. pombe viability. On the whole, our data indicate that the Eso1 interaction with Pol5 which acetylates Pol5 protein is essential for S. pombe viability.
Authors	Zhiming Chen, Hongshi Cao, Yingqiang Lu, Qiang Ren, Liankun Sun
Journal	International journal of molecular medicine (Int J Mol Med) Vol. 40 Issue 6 Pg. 1907-1913 (Dec 2017) ISSN: 1791-244X [Electronic] Greece
PMID	29039458 (Publication Type: Journal Article)
Chemical References	Cell Cycle Proteins Eso1 protein, S pombe Schizosaccharomyces pombe Proteins CDC2 Protein Kinase cdc2 protein, S pombe DNA-Directed DNA Polymerase Rad30 protein Minichromosome Maintenance Complex Component 4 mcm4 protein, S pombe
Topics	Acetylation Amino Acid Sequence (genetics) CDC2 Protein Kinase (genetics) Cell Cycle Proteins (genetics) Cell Survival (genetics) Chromatids (genetics) DNA-Directed DNA Polymerase (genetics) Minichromosome Maintenance Complex Component 4 (genetics) Protein Binding Schizosaccharomyces (genetics) Schizosaccharomyces pombe Proteins (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!