Abstract |
Autosomal dominant polycystic kidney disease ( ADPKD) constitutes the most inherited kidney disease. Mutations in the PKD1 and PKD2 genes, encoding the polycystin 1 and polycystin 2 Ca2+ ion channels, respectively, result in tubular epithelial cell-derived renal cysts. Recent clinical studies demonstrate oxidative stress to be present early in ADPKD. Mitochondria comprise the primary reactive oxygen species source and also their main effector target; however, the pathophysiological role of mitochondria in ADPKD remains uncharacterized. To clarify this function, we examined the mitochondria of cyst-lining cells in ADPKD model mice (Ksp-Cre PKD1flox/flox) and rats (Han:SPRD Cy/+), demonstrating obvious tubular cell morphological abnormalities. Notably, the mitochondrial DNA copy number and peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) expression were decreased in ADPKD model animal kidneys, with PGC-1α expression inversely correlated with oxidative stress levels. Consistent with these findings, human ADPKD cyst-derived cells with heterozygous and homozygous PKD1 mutation exhibited morphological and functional abnormalities, including increased mitochondrial superoxide. Furthermore, PGC-1α expression was suppressed by decreased intracellular Ca2+ levels via calcineurin, p38 mitogen-activated protein kinase (MAPK), and nitric oxide synthase deactivation. Moreover, the mitochondrion-specific antioxidant MitoQuinone ( MitoQ) reduced intracellular superoxide and inhibited cyst epithelial cell proliferation through extracellular signal-related kinase/MAPK inactivation. Collectively, these results indicate that mitochondrial abnormalities facilitate cyst formation in ADPKD.
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Authors | Yu Ishimoto, Reiko Inagi, Daisuke Yoshihara, Masanori Kugita, Shizuko Nagao, Akira Shimizu, Norihiko Takeda, Masaki Wake, Kenjiro Honda, Jing Zhou, Masaomi Nangaku |
Journal | Molecular and cellular biology
(Mol Cell Biol)
Vol. 37
Issue 24
(12 15 2017)
ISSN: 1098-5549 [Electronic] United States |
PMID | 28993480
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | Copyright © 2017 Ishimoto et al. |
Chemical References |
- TRPP Cation Channels
- polycystic kidney disease 1 protein
- polycystic kidney disease 2 protein
- Superoxides
- Nitric Oxide Synthase
- Extracellular Signal-Regulated MAP Kinases
- p38 Mitogen-Activated Protein Kinases
- Calcineurin
- Calcium
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Topics |
- Animals
- Calcineurin
(metabolism)
- Calcium
(metabolism)
- Cell Proliferation
(physiology)
- Cells, Cultured
- Cysts
(metabolism, pathology)
- Disease Models, Animal
- Epithelial Cells
(metabolism, pathology)
- Extracellular Signal-Regulated MAP Kinases
- Humans
- Kidney
(metabolism, pathology)
- Mice
- Mitochondria
(metabolism, pathology)
- Mutation
(genetics)
- Nitric Oxide Synthase
(metabolism)
- Polycystic Kidney, Autosomal Dominant
(metabolism, pathology)
- Rats
- Signal Transduction
(physiology)
- Superoxides
(metabolism)
- TRPP Cation Channels
(metabolism)
- p38 Mitogen-Activated Protein Kinases
(metabolism)
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