To investigate the etiopathogenesis of
fatty liver hemorrhagic syndrome (
FLHS) and the protective effects of soybean
lecithin against
FLHS in laying hens, 135 healthy 300-day-old Hyline laying hens were randomly divided into groups: control (group 1), diseased (group 2), and protected (group 3). Each group contained 45 layers with 3 replicates. The birds in these 3 groups were fed a control diet, a high-energy/low-
protein (HELP) diet or the HELP diet supplemented with 3% soybean
lecithin instead of maize. The fat percent in the liver was calculated. Histopathological changes in the liver were determined by staining, and the
mRNA expression levels of apolipoproteinA I (
apoA I) and
apolipoprotein B100 (apoB100) in the liver were determined by RT-PCR. The results showed that the fat percent in the liver of group 2 was much higher (P < 0.01) than that of group 1 and group 2 on d 30 and 60. The histology of the liver in group 2 on d 30 and 60 displayed various degrees of liver lesions, while the hepatocytes showed a normal structure in group 3 with mild microvesicular steatosis in the liver cell on d 30 and 60. The
mRNA expression levels of
apoA I and apoB100 in the livers were variable throughout the experiment. The expression level of
apoA I in group 2 significantly decreased on d 60 (P < 0.05); the expression level of apoB100 slightly increased on d 30 in group 2, while it sharply decreased on d 60. Compared to group 1, the expression level of apoB100 showed no significant difference in group 3 (P < 0.05). This study indicated that
FLHS induced pathological changes and abnormal expression of
apoA I and apoB100 in the livers of laying hens and that soybean
lecithin alleviated these abnormal changes.