Abstract |
A significant inflammatory reaction in the rat conjunctiva followed the subconjunctival injection of synthetic platelet activating factor (PAF) in doses which ranged from 10 ng to 1 microgram, an inflammatory response being evaluated as the increase in both tissue weight and extravasation of Evans blue dye in the conjunctival tissue. Inflammation was still present 6 h after the injection of 0.1 microgram of PAF. Orally administered indomethacin or BW 755C failed to alter the response to 0.1 microgram of PAF. In contrast, the PAF-induced inflammation was blocked by the oral administration of the PAF receptor antagonist, L-652,731, a dose as low as 5 mg kg-1 eliciting a significant inhibition. The topical administration of L-652,731, (two doses of 100 micrograms as a 1% suspension), elicited a slight, but significant blockade of 23%. Its antagonistic action was more striking when it was co-injected subconjunctivally with 0.1 microgram of PAF, a dose as low as 3 micrograms evoking a significant blockade. The topical administration of 0.1 microgram of PAF did not elicit a significant inflammatory reaction and this contrasts with the results obtained after its subconjunctival injection.
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Authors | P D Gautheron, L Coulbault, M F Sugrue |
Journal | The Journal of pharmacy and pharmacology
(J Pharm Pharmacol)
Vol. 39
Issue 10
Pg. 857-9
(Oct 1987)
ISSN: 0022-3573 [Print] England |
PMID | 2891830
(Publication Type: Journal Article)
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Chemical References |
- Furans
- Platelet Activating Factor
- Evans Blue
- 2,5-bis(3,4,5-trimethoxyphenyl)tetrahydrofuran
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Topics |
- Animals
- Conjunctivitis
(chemically induced, physiopathology)
- Evans Blue
- Furans
(pharmacology)
- Organ Size
(drug effects)
- Platelet Activating Factor
(antagonists & inhibitors)
- Rats
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