NLRC5 promotes cell proliferation via regulating the NF-κB signaling pathway in Rheumatoid arthritis.

Abstract

Rheumatoid arthritis (RA) is a chronic autoimmune inflammatory disease and the pathogenesis remains unclear. Previous studies suggested that fibroblast-like synoviocytes (FLSs) play an important role in RA pathogenesis, including the injury of cartilage, the hyperplasia of the synovium and the release of inflammatory cytokines. We used complete Freund's adjuvant (CFA) induced rats as animal models for studying the RA pathogenesis. NLRC5 as the largest member of the NLR family has been reported to play a critical role in regulating immune responses. Increasing evidence suggests that NLRC5 is an pivotal negative modulator of inflammatory pathways. We investigated the mechanisms and signaling pathways of NLRC5 in RA progression. Significantly increased expression of NLRC5 was found in AA rats synovial tissues and cells. And high expression of inflammatory cytokine and cell proliferation of FLSs accompanied with NLRC5 overexpression, but inhibited in cells with NLRC5 silencing treatment. Interestingly, we found that overexpression of NLRC5 also coordinated the activation of NF-κB signaling pathway. These results suggested that NLRC5 promotes RA progression via the NF-κB signaling pathway potentially.

• Authors: Ya-Ru Liu, Xing Yan, Hai-Xia Yu, Yao Yao, Jie-Quan Wang, Xiao-Feng Li, Ruo-Nan Chen, Qing-Qing Xu, Tao-Tao Ma, Cheng Huang, Jun Li
• Journal: Molecular immunology (Mol Immunol) Vol. 91 Pg. 24-34 (11 2017) ISSN: 1872-9142 [Electronic] England
• PMID: 28865311 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017. Published by Elsevier Ltd.

Chemical References

• Cytokines
• Intracellular Signaling Peptides and Proteins
• NF-kappa B
• Freund's Adjuvant

Topics

• Animals
• Arthritis, Rheumatoid (chemically induced, genetics, immunology)
• Cell Proliferation
• Cytokines (genetics, immunology)
• Female
• Freund's Adjuvant (adverse effects, pharmacology)
• Gene Expression Regulation (drug effects, genetics, immunology)
• Intracellular Signaling Peptides and Proteins (genetics, immunology)
• NF-kappa B (genetics, immunology)
• Rats
• Rats, Sprague-Dawley
• Signal Transduction (drug effects, genetics, immunology)
• Synovial Membrane (immunology, pathology)