Effect of curcumin on glycerol-induced acute kidney injury in rats.

Abstract

The aim of this study was to investigate the protective role and underlying mechanisms of curcumin on glycerol-induced acute kidney injury (AKI) in rats. Glycerol (10 ml/kg BW, 50% v/v in sterile saline, i.m.) was used to induce AKI, followed by curcumin (200 mg/kg/day, p.o.) administration for 3 days. To confirm renal damage and the effects of curcumin on AKI, serum BUN, Scr, and CK as well as renal SOD, MDA, GSH-Px were measured. Additionally, morphological changes were identified by H&E staining and transmission electron microscopy. The expression of several factors including chemotactic factor MCP-1, proinflammatory cytokines including TNF-α and IL-6, as well as the kidney injury markers, as Kim-1 and Lipocalin-2 were also assessed using q-PCR. Finally, cell apoptosis in renal tissue was detected using in situ TUNEL apoptosis fluorescence staining and expression of proteins associated with apoptotic, oxidative stress and lipid oxidative related signaling pathways were detected using immunohistochemical staining and western blot. The results showed that curcumin exerts renoprotective effects by inhibiting oxidative stress in rhabdomyolysis-induced AKI through regulation of the AMPK and Nrf2/HO-1 signaling pathways, and also ameliorated RM-associated renal injury and cell apoptosis by activating the PI3K/Akt pathway.

• Authors: Jindao Wu, Xiongxiong Pan, Heling Fu, Yuan Zheng, Youjin Dai, Yuan Yin, Qin Chen, Qingting Hao, Dan Bao, Daorong Hou
• Journal: Scientific reports (Sci Rep) Vol. 7 Issue 1 Pg. 10114 (08 31 2017) ISSN: 2045-2322 [Electronic] England
• PMID: 28860665 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Antioxidants
• Cell Adhesion Molecules
• Havcr1protein, rat
• Lipocalin-2
• NF-E2-Related Factor 2
• Nfe2l2 protein, rat
• Heme Oxygenase-1
• Protein Kinases
• AMP-Activated Protein Kinase Kinases
• Curcumin
• Glycerol

Topics

• AMP-Activated Protein Kinase Kinases
• Acute Kidney Injury (drug therapy, etiology)
• Animals
• Anti-Inflammatory Agents, Non-Steroidal (therapeutic use)
• Antioxidants (therapeutic use)
• Apoptosis
• Cell Adhesion Molecules (metabolism)
• Curcumin (therapeutic use)
• Female
• Glycerol (toxicity)
• Heme Oxygenase-1 (metabolism)
• Kidney (metabolism)
• Lipocalin-2 (metabolism)
• NF-E2-Related Factor 2 (metabolism)
• Oxidative Stress
• Protein Kinases (metabolism)
• Rats
• Rats, Sprague-Dawley