Nicotinamide mononucleotide and related metabolites induce disease resistance against fungal phytopathogens in Arabidopsis and barley.

Abstract

Nicotinamide mononucleotide (NMN), a precursor of nicotinamide adenine dinucleotide (NAD), is known to act as a functional molecule in animals, whereas its function in plants is largely unknown. In this study, we found that NMN accumulated in barley cultivars resistant to phytopathogenic fungal Fusarium species. Although NMN does not possess antifungal activity, pretreatment with NMN and related metabolites enhanced disease resistance to Fusarium graminearum in Arabidopsis leaves and flowers and in barley spikes. The NMN-induced Fusarium resistance was accompanied by activation of the salicylic acid-mediated signalling pathway and repression of the jasmonic acid/ethylene-dependent signalling pathways in Arabidopsis. Since NMN-induced disease resistance was also observed in the SA-deficient sid2 mutant, an SA-independent signalling pathway also regulated the enhanced resistance induced by NMN. Compared with NMN, NAD and NADP, nicotinamide pretreatment had minor effects on resistance to F. graminearum. Constitutive expression of the NMNAT gene, which encodes a rate-limiting enzyme for NAD biosynthesis, resulted in enhanced disease resistance in Arabidopsis. Thus, modifying the content of NAD-related metabolites can be used to optimize the defence signalling pathways activated in response to F. graminearum and facilitates the control of disease injury and mycotoxin accumulation in plants.

• Authors: Akihiro Miwa, Yuji Sawada, Daisuke Tamaoki, Masami Yokota Hirai, Makoto Kimura, Kazuhiro Sato, Takumi Nishiuchi
• Journal: Scientific reports (Sci Rep) Vol. 7 Issue 1 Pg. 6389 (07 25 2017) ISSN: 2045-2322 [Electronic] England
• PMID: 28743869 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Plant Proteins
• NAD
• Nicotinamide Mononucleotide
• Nicotinamide-Nucleotide Adenylyltransferase
• Salicylic Acid

Topics

• Arabidopsis (genetics, microbiology)
• Disease Resistance
• Flowers (microbiology)
• Fusarium (drug effects)
• Hordeum (genetics, microbiology)
• NAD (metabolism, pharmacology)
• Nicotinamide Mononucleotide (chemistry, metabolism, pharmacology)
• Nicotinamide-Nucleotide Adenylyltransferase (genetics)
• Plant Diseases (prevention & control)
• Plant Leaves (microbiology)
• Plant Proteins (genetics)
• Salicylic Acid (metabolism)
• Signal Transduction