Salivary C-reactive protein, mean platelet volume and neutrophil lymphocyte ratio as diagnostic markers for neonatal sepsis.

Abstract

OBJECTIVE:

To assess the applicability of salivary C-reactive protein, mean platelet volume, neutrophil-lymphocyte ratio, and platelet lymphocyte ratio in the diagnosis of neonatal sepsis.

METHODS:

Prospective case-control study of 70 full-term neonates, 35 with sepsis (20 with proven sepsis and 15 with clinical sepsis) and 35 healthy controls. Serum and salivary C-reactive protein concentrations were measured by enzyme-linked immunosorbent assay while mean platelet volume, neutrophil-lymphocyte ratio, and platelet lymphocyte ratio were measured by automated blood cell counter.

RESULTS:

This study showed statistically significant difference of mean salivary C-reactive protein between septic neonates and controls (12.0±4.6ng/L vs. 2.8±1.2ng/L) respectively. At a cut-off point of 3.48ng/L, salivary C-reactive protein showed 94.3% sensitivity and 80% specificity. Salivary C-reactive protein also showed good predictive accuracy for predicting elevated serum C-reactive protein values in septic neonates. Mean platelet volume and neutrophil-lymphocyte ratio showed significant difference between septic neonates and controls (10.2±1.2fL vs.8.0±0.5fL; 2.9±1.7 vs. 1.6±0.4, respectively). At a cut-off point of 10.2fL, mean platelet volume presented 80% sensitivity and specificity. At a cut-off point of 2.7, neutrophil-lymphocyte ratio presented 80% sensitivity and 57.1% specificity.

CONCLUSION:

This study provides support for further studies on the usefulness of salivary C-reactive protein, mean platelet volume, and neutrophil-lymphocyte ratio as diagnostic markers for neonatal sepsis.

• Authors: Ahmed Omran, Abdallah Maaroof, Mai H S Mohammad, Amina Abdelwahab
• Journal: Jornal de pediatria (J Pediatr (Rio J)) 2018 Jan - Feb Vol. 94 Issue 1 Pg. 82-87 ISSN: 1678-4782 [Electronic] Brazil
• PMID: 28734690 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Sociedade Brasileira de Pediatria. Published by Elsevier Editora Ltda. All rights reserved.

Chemical References

• Biomarkers
• C-Reactive Protein

Topics

• Biomarkers (analysis)
• C-Reactive Protein (analysis)
• Case-Control Studies
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Infant, Newborn
• Lymphocyte Count
• Male
• Mean Platelet Volume
• Neonatal Sepsis (diagnosis)
• Neutrophils (cytology)
• Prospective Studies
• ROC Curve
• Saliva (chemistry)
• Sensitivity and Specificity