Abstract |
The formation of the allotetraploid hybrid lineage (4nAT) encompasses both distant hybridization and polyploidization processes. The allotetraploid offspring have two sets of sub-genomes inherited from both parental species, and therefore, it is important to explore its genetic structure. Herein, we construct a bacterial artificial chromosome library of allotetraploids, and then sequence and analyze the full-length sequences of 19 bacterial artificial chromosomes. Sixty-eight DNA chimeras are identified, which are divided into four models according to the distribution of the genomic DNA derived from the parents. Among the 68 genetic chimeras, 44 (64.71%) are linked to tandem repeats (TRs) and 23 (33.82%) are linked to transposable elements ( TEs). The chimeras linked to TRs are related to slipped-strand mispairing and double-strand break repair while the chimeras linked to TEs benefit from the intervention of recombinases. In addition, TRs and TEs can also result in insertions/deletions of DNA segments. We conclude that DNA chimeras accompanied by TRs and TEs coordinate a balance between the sub-genomes derived from the parents. It is the first report on the relationship between formation of the DNA chimeras and TRs and TEs in the polyploid animals.
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Authors | Lihai Ye, Ni Jiao, Xiaojun Tang, Yiyi Chen, Xiaolan Ye, Li Ren, Fangzhou Hu, Shi Wang, Ming Wen, Chun Zhang, Min Tao, Shaojun Liu |
Journal | Marine biotechnology (New York, N.Y.)
(Mar Biotechnol (NY))
Vol. 19
Issue 4
Pg. 401-409
(Aug 2017)
ISSN: 1436-2236 [Electronic] United States |
PMID | 28681105
(Publication Type: Journal Article)
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Chemical References |
- DNA Transposable Elements
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Topics |
- Animals
- Carps
(genetics)
- Chromosomes, Artificial, Bacterial
- DNA Transposable Elements
- Gene Library
- Hybridization, Genetic
- INDEL Mutation
- Sequence Analysis, DNA
- Tandem Repeat Sequences
- Tetraploidy
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