Protection of Lipopolysaccharide (LPS) Preconditioning against Endotoxin-Induced Uveitis (EIU) in Rats is Associated with Overexpression of Interleukin-1 Receptor-Associated Kinase M (IRAK-M).

Abstract

PURPOSE:

To investigate the protective effect of LPS preconditioning against EIU in rats.

METHODS:

EIU in Wistar rats was developed by subcutaneous injection of LPS (200 μg). Lower dose of LPS (0.1 mg/kg, intraperitoneally) or its carrier was injected daily for five days before EIU induction. Twenty-four hours after EIU, eyes were examined and then enucleated. The degree of inflammatory reaction was determined by routine histological examinations. Real-time RT-PCR and Western blot were used to determine the activation of NF-kB and expression of IRAK-1, IRAK-4, and IRAK-M Results: Repeated pre-administration of LPS induced a significant reduction in ocular inflammation and the expression of NF-κb p65 in neurons. The expression of IRAK-1 and IRAK-4 was suppressed in endotoxin tolerance group, whereas IRAK-M was increased.

CONCLUSIONS:

Endotoxin tolerance has a protective effect against EIU, and upregulation of IRAK-M through TLR-signaling pathway is one of the most likely candidates to be involved in the observed phenomenon.

• Authors: Shuo Yu, Xinli Liu, Nan Zhang, Shuo Yang, Cui Mao, Shilan Feng, Hong Lu
• Journal: Ocular immunology and inflammation (Ocul Immunol Inflamm) Vol. 26 Issue 6 Pg. 943-950 ( 2018) ISSN: 1744-5078 [Electronic] England
• PMID: 28609207 (Publication Type: Journal Article)

Chemical References

• Endotoxins
• Lipopolysaccharides
• RNA
• IRAK1 protein, rat
• Interleukin-1 Receptor-Associated Kinases

Topics

• Animals
• Aqueous Humor (enzymology)
• Blotting, Western
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Endotoxins (toxicity)
• Gene Expression Regulation
• Interleukin-1 Receptor-Associated Kinases (biosynthesis, genetics)
• Lipopolysaccharides (administration & dosage)
• Male
• RNA (genetics)
• Rats
• Rats, Wistar
• Real-Time Polymerase Chain Reaction
• Uveitis (chemically induced, genetics, prevention & control)