The effect of caffeic acid phenethyl ester (CAPE) on tympanosclerosis.

Abstract

OBJECTIVE:

To investigate the effects of caffeic acid phenethyl ester (CAPE) on tympanosclerosis.

MATERIALS AND METHODS:

Thirty-two male Sprague Dawley rats were separated into 4 groups as CAPE (n = 10), alcohol (n = 10), control (n = 8) and normal (n = 4) groups. All tympanic membranes except normal group were myringotomised and type 3 Streptococcus pneumoniae strains was injected into their middle ears. Myringotomies were repeated for 5 weeks. Intraperitoneal (i.p) CAPE were administrated to the CAPE group at 10 μmol/kg/day and 10% ethyl alcohol administrated to the alcohol group for 5 weeks. The control group were left untreated. Findings of myringosclerosis were recorded by otomicroscope at sixth week. Then, all rats were sacrificed and tympanic membrane thickness and severity of middle ear mucosal inflammation evaluated histopathalogically.

RESULTS:

Severity of myringosclerosis was significantly higher in the alcohol and control groups compared to the CAPE group (p < 0.001), but was not significant when alcohol and control groups were compared (p = 0.17). The tympanic membrane thickness measured in the alcohol and control groups were significantly higher compared to the CAPE group (p < 0.001), but was not significant when alcohol and control groups were compared (p = 0.17). The severity of inflammation in the middle ear mucosa was significantly higher in the alcohol and control groups compared to the CAPE group (respectively, p < 0.001, p = 0.03). The severity of inflammation in the middle ear mucosa was not significant between alcohol and control groups (p = 0.30).

CONCLUSION:

CAPE has anti-inflammatory and antioxidant effects on the development of MS in myringotomized rats, so reduces the severity of tympanosclerosis.

• Authors: İbrahim Ağrı, Arzu Erdal Ağrı, Mehmet Eser Sancaktar, Asude Ünal, Erkan Can, Mustafa Bakırtaş, Fatih Yılmaz, Mehmet Derya Demirağ, Recep Ünal
• Journal: International journal of pediatric otorhinolaryngology (Int J Pediatr Otorhinolaryngol) Vol. 95 Pg. 127-132 (Apr 2017) ISSN: 1872-8464 [Electronic] Ireland
• PMID: 28576521 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Caffeic Acids
• caffeic acid phenethyl ester
• Phenylethyl Alcohol

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Caffeic Acids (pharmacology)
• Inflammation (drug therapy)
• Male
• Middle Ear Ventilation
• Myringosclerosis (drug therapy)
• Phenylethyl Alcohol (analogs & derivatives, pharmacology)
• Rats
• Rats, Sprague-Dawley
• Tympanic Membrane (drug effects, pathology)