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Bentonite modified with zinc enhances aflatoxin B1 adsorption and increase survival of fibroblasts (3T3) and epithelial colorectal adenocarcinoma cells (Caco-2).

Abstract
Bentonites are commonly used as feed additives to reduce the bioavailability and thus the toxicity of aflatoxins by adsorbing the toxins in the gastrointestinal tract. Aflatoxins are particular harmful mycotoxins mainly found in areas with hot and humid climates. They occur in food and feedstuff as a result of fungal contamination before and after harvest. The aim of this study was to modify Brazilian bentonite clay by incorporation of zinc (Zn) ions in order to increase the adsorption capacity and consequently reduce the toxicity of aflatoxins. The significance of Zn intercalating conditions such as concentration, temperature and reaction time were investigated. Our results showed that the Zn treatment of the bentonite increased the aflatoxin B1 (AFB1) adsorption and that Zn concentration had a negative effect. Indeed, temperature and time had no significant effect in the binding capacity. The modified bentonite (Zn-Bent1) was not cytotoxic to either fibroblasts (3T3) nor epithelial colorectal adenocarcinoma cells (Caco-2) cell lines. Interestingly, Zn-Bent1 has higher protective effect against AFB1 induced cytotoxicity than the unmodified bentonite. In conclusion, the Zn modified bentonite, Zn-Bent1, represent an improved tool to prevent aflatoxicosis in animals fed on AFB1 contaminated feed.
AuthorsJanaína Nones, Anita Solhaug, Gunnar Sundstøl Eriksen, Domingos Lusitâneo Pier Macuvele, Anicleto Poli, Cíntia Soares, Andrea Gonçalves Trentin, Humberto Gracher Riella, Jader Nones
JournalJournal of hazardous materials (J Hazard Mater) Vol. 337 Pg. 80-89 (Sep 05 2017) ISSN: 1873-3336 [Electronic] Netherlands
PMID28511044 (Publication Type: Journal Article)
CopyrightCopyright © 2017 Elsevier B.V. All rights reserved.
Chemical References
  • Bentonite
  • Aflatoxin B1
  • Zinc
Topics
  • 3T3 Cells
  • Adsorption
  • Aflatoxin B1 (chemistry, isolation & purification, poisoning)
  • Animal Feed (analysis)
  • Animals
  • Bentonite (chemistry, pharmacology)
  • Caco-2 Cells
  • Cell Survival (drug effects)
  • Food Contamination (analysis)
  • Humans
  • Mice
  • Microscopy, Electron, Scanning
  • Poisoning (prevention & control, veterinary)
  • Spectroscopy, Fourier Transform Infrared
  • Zinc (chemistry)

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