Abstract |
Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) are known to have strong anti-inflammatory effects. In the present study, we investigated the protective effects of ω-3 PUFAs on experimentally induced murine colitis. Intrarectal administration of 2.5% 2,4,6-trinitrobenzene sulfonic acid (TNBS) caused inflammation in the colon of wild type mice, but this was less severe in fat-1 transgenic mice that constitutively produce ω-3 PUFAs from ω-6 PUFAs. The intraperitoneal administration of docosahexaenoic acid (DHA), a representative ω-3 PUFA, was also protective against TNBS-induced murine colitis. In addition, endogenously formed and exogenously introduced ω-3 PUFAs attenuated the production of malondialdehyde and 4-hydroxynonenal in the colon of TNBS-treated mice. The effective protection against inflammatory and oxidative colonic tissue damages in fat-1 and DHA-treated mice was associated with suppression of NF-κB activation and cyclooxygenase-2 expression and with elevated activation of Nrf2 and upregulation of its target gene, heme oxygenase-1. Taken together, these results provide mechanistic basis of protective action of ω-3 fatty PUFAs against experimental colitis.
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Authors | Hye-Won Yum, Jing X Kang, Ki Baik Hahm, Young-Joon Surh |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 487
Issue 4
Pg. 847-855
(06 10 2017)
ISSN: 1090-2104 [Electronic] United States |
PMID | 28456627
(Publication Type: Journal Article)
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Copyright | Copyright © 2017. Published by Elsevier Inc. |
Chemical References |
- Cadherins
- Fatty Acids, Omega-3
- fat1 protein, mouse
- Docosahexaenoic Acids
- Trinitrobenzenesulfonic Acid
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Topics |
- Animals
- Cadherins
(metabolism)
- Colitis
(chemically induced, prevention & control)
- Docosahexaenoic Acids
(administration & dosage, pharmacology)
- Fatty Acids, Omega-3
(biosynthesis)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Trinitrobenzenesulfonic Acid
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