Watsonianone A from Rhodomyrtus tomentosa Fruit Attenuates Respiratory-Syncytial-Virus-Induced Inflammation In Vitro.

Abstract

Respiratory syncytial virus (RSV) is one of the most common respiratory pathogens. Immoderate inflammation plays a great role in causing RSV-induced diseases. In the present study, watsonianone A, isolated from the fruit of Rhodomyrtus tomentosa (Ait.) Hassk, was found to show a good inhibitory effect on RSV-induced NO production, with a half-maximal inhibitory concentration of 37.2 ± 1.6 μM. Enzyme-linked immunosorbent assay and fluorescence quantitative polymerase chain reaction analyses indicated that watsonianone A markedly reduced both mRNA and protein levels of tumor necrosis factor α, interleukin 6, and monocyte chemoattractant protein 1 in RSV-infected RAW264.7 cells. Mechanistically, watsonianone A inhibited nuclear factor κB (NF-κB) activation by suppressing IκBα phosphorylation. Further analysis revealed that watsonianone A activated the thioredoxin system and decreased intracellular reactive oxygen species (ROS) levels, which are closely associated with NF-κB activation in RSV-infected cells. These results reveal that watsonianone A can attenuate RSV-induced inflammation via the suppression of ROS-sensitive inflammatory signaling.

• Authors: Ling Zhuang, Li-Feng Chen, Yu-Bo Zhang, Zhong Liu, Xu-Hui Xiao, Wei Tang, Guo-Cai Wang, Wen-Jun Song, Yao-Lan Li, Man-Mei Li
• Journal: Journal of agricultural and food chemistry (J Agric Food Chem) Vol. 65 Issue 17 Pg. 3481-3489 (May 03 2017) ISSN: 1520-5118 [Electronic] United States
• PMID: 28436225 (Publication Type: Journal Article)

Chemical References

• Cyclohexanones
• I-kappa B Proteins
• IL6 protein, human
• Interleukin-6
• NF-kappa B
• Plant Extracts
• Reactive Oxygen Species
• Tumor Necrosis Factor-alpha
• watsonianone A

Topics

• Cyclohexanones (pharmacology)
• Fruit (chemistry)
• Humans
• I-kappa B Proteins (genetics, immunology)
• Interleukin-6 (genetics, immunology)
• Myrtaceae (chemistry)
• NF-kappa B (genetics, immunology)
• Plant Extracts (pharmacology)
• Reactive Oxygen Species (immunology)
• Respiratory Syncytial Virus Infections (drug therapy, immunology, virology)
• Respiratory Syncytial Viruses (drug effects, physiology)
• Tumor Necrosis Factor-alpha (genetics, immunology)