Effects of Agaricus blazei Murill Polysaccharide on Cadmium Poisoning on the MDA5 Signaling Pathway and Antioxidant Function of Chicken Peripheral Blood Lymphocytes.

Abstract

This experimental study investigated the effect of Agaricus blazei Murill polysaccharide (ABP) on cadmium (Cd) poisoning on the melanoma differentiation-associated gene 5 (MDA5) signaling pathway and antioxidant function of peripheral blood lymphocytes (PBLs) in chickens. The experiments were divided into four groups: 7-day-old chickens with normal saline (0.2 mL single/day), Cd (140 mg/kg), ABP (30 mg/mL, 0.2 mL single/day), and Cd + ABP(140 mg/kg/day + 0.2 mL ABP). Peripheral blood was collected on the 20th, 40th, and 60th days for each group, and PBLs were separated. We attempted to detect the expression of MDA5, downstream signaling molecules, and convergence protein (interferon promoter-stimulating factor 1); transcription factors (IRF3 and NF-κB); the content of cytokines (IL-1β, IL-6, TNF-α, and IFN-β) in PBLs; and the antioxidant index of superoxide dismutase (SOD), malondialdhyde (MDA), and glutathione peroxidase (GSH-Px). The results showed that ABP can reduce the accumulation of Cd in the peripheral blood of chickens; reduce the expression of MDA5 and downstream signaling molecules; and reduce the content of IL-1β, IL-6, TNF-α, and IFN-β in PBLs of chickens. The activity of antioxidant enzymes (SOD and GSH-Px) significantly increased, and the content of MDA decreased. These results showed that they have a certain protective effect of ABP on Cd poisoning in chicken PBLs caused by injury.

• Authors: Ai Lv, Ming Ge, Xuequan Hu, Wenjing Liu, Guangxing Li, Ruili Zhang
• Journal: Biological trace element research (Biol Trace Elem Res) Vol. 181 Issue 1 Pg. 122-132 (Jan 2018) ISSN: 1559-0720 [Electronic] United States
• PMID: 28432527 (Publication Type: Journal Article)

Chemical References

• Antioxidants
• Polysaccharides
• Cadmium
• Glutathione Peroxidase
• Superoxide Dismutase
• Interferon-Induced Helicase, IFIH1

Topics

• Agaricus
• Animals
• Antioxidants (chemistry, pharmacology)
• Cadmium (analysis)
• Cadmium Poisoning (drug therapy)
• Chickens
• Glutathione Peroxidase (metabolism)
• Interferon-Induced Helicase, IFIH1 (antagonists & inhibitors, genetics)
• Lymphocytes (drug effects, metabolism)
• Polysaccharides (chemistry, pharmacology)
• Signal Transduction (drug effects)
• Superoxide Dismutase (metabolism)