Increased alloimmunisation and transfusion reaction reporting in patients with solid-phase panreactivity.

Abstract

BACKGROUND:

Automated solid-phase antibody screening uses red blood cell (RBC) membranes immobilised on polystyrene test wells to detect RBC specific antibodies. Despite its time-saving and labour-saving benefits, this method produces a higher rate of nonspecific reactivity compared with manual screening. Solid-phase panreactivity (SPP) is characterised by panreactivity (ie, all test cells reacting) in solid-phase testing accompanied by a negative autocontrol and a lack of reactivity when the same screening cells are tested in tube. The mechanisms underlying SPP and its clinical significance remain unclear. The goals of this study were to describe the prevalence of SPP at our institution and determine the alloimmunisation and transfusion reaction rates within this population.

METHODS:

Data were collected on all patients undergoing type and screen testing over a 6-year period. Study patients undergoing subsequent transfusion were evaluated for reported transfusion reactions and development of new alloantibodies.

RESULTS:

Of the 76 051 patients studied, 0.7% demonstrated SPP of which 11% developed new alloantibodies. The transfusion reaction reporting rate among patients with SPP was 2%.

CONCLUSIONS:

Our data suggest that patients with SPP have higher rates of reported transfusion reactions and alloantibody development compared with those without SPP.

• Authors: Andrea M Olofson, Rachael M Chandler, Cynthia R Marx-Wood, Craig A Babcock, Nancy M Dunbar
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 70 Issue 11 Pg. 981-983 (Nov 2017) ISSN: 1472-4146 [Electronic] England
• PMID: 28424235 (Publication Type: Journal Article)
• Copyright: Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/.

Chemical References

• Isoantibodies
• Isoantigens

Topics

• Automation, Laboratory
• Blood Grouping and Crossmatching (methods)
• Erythrocyte Transfusion (adverse effects)
• Erythrocytes (immunology)
• Histocompatibility
• Humans
• Isoantibodies (blood)
• Isoantigens (blood)
• Predictive Value of Tests
• Reproducibility of Results
• Retrospective Studies
• Risk Factors
• Transfusion Reaction (blood, etiology, immunology)
• Workload