Transfection of Primary Human Skin Fibroblasts for Peroxisomal Studies.

Abstract

Functional studies with primary human skin fibroblasts from patients with a peroxisomal disorder often require efficient transfection with plasmids to correct the genetic defect or to express heterologous reporter proteins. Here, we describe a protocol we commonly use for efficient nonviral transfection of primary human skin fibroblasts.

• Authors: Janet Koster, Hans R Waterham
• Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol) Vol. 1595 Pg. 63-67 ( 2017) ISSN: 1940-6029 [Electronic] United States
• PMID: 28409452 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Fibroblasts (metabolism)
• Humans
• Peroxisomes (metabolism)
• Primary Cell Culture
• Skin (cytology)
• Transfection