shRNAs targeting either the glycoprotein or polymerase genes inhibit Viral haemorrhagic septicaemia virus replication in zebrafish ZF4 cells.

Abstract	Viral haemorrhagic septicaemia virus (VHSV) represents an important disease of finfish. To explore the potential of shRNAs to combat this disease nucleotide sequences of either the VHSV glycoprotein (G) or polymerase (L) gene were targeted. To test their function, shRNAs were expressed in zebrafish epithelial ZF-4 cells utilizing the zebrafish U6-2 promoter. Five of the six shRNA molecules successfully reduced VHSV replication by between 2 and 4 logs in titre relative to an irrelevant control shRNA at all MOIs and also reduced viral CPE at the highest MOI. To ensure that observed reductions in viral titre were dependent on shRNA silencing, potential non-specific antiviral responses were assessed. Only the ineffective shRNA, which formed an improper hairpin when analysed in silico, induced an antiviral response as measured by induction of interferon (ifnphi1) and Mx (MxA) genes. These results represent an important preliminary step in the generation of transgenic zebrafish resistant to VHSV.
Authors	Brian D Clarke, Kenneth A McColl, Alister C Ward, Timothy J Doran
Journal	Antiviral research (Antiviral Res) Vol. 141 Pg. 124-132 (05 2017) ISSN: 1872-9096 [Electronic] Netherlands
PMID	28237822 (Publication Type: Journal Article)
Copyright	Copyright © 2017 Elsevier B.V. All rights reserved.
Chemical References	G protein, viral hemorrhagic septicemia virus RNA, Small Interfering Viral Envelope Proteins Zebrafish Proteins interferon phi1, zebrafish Interferons RNA-Dependent RNA Polymerase
Topics	Animals Cell Line Cytopathogenic Effect, Viral DNA Replication (drug effects) Electroporation Fish Diseases (prevention & control) Hemorrhagic Septicemia, Viral (prevention & control) Interferons (genetics) Novirhabdovirus (drug effects, enzymology, pathogenicity, physiology) Promoter Regions, Genetic RNA Interference RNA, Small Interfering (genetics) RNA-Dependent RNA Polymerase (genetics) Transfection Viral Envelope Proteins (genetics) Virus Replication (drug effects) Zebrafish Zebrafish Proteins (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.

Realize the full power of the drug-disease research graph!