The competing endogenous RNA network of CYP4Z1 and pseudogene CYP4Z2P exerts an anti-apoptotic function in breast cancer.

Abstract

The competing endogenous RNA network (ceRNET) is involved in tumorigenesis and has become a hot spot of research. The ceRNET between CYP4Z1 and the pseudogene CYP4Z2P promotes angiogenesis and mediates tamoxifen resistance in breast cancer. Nevertheless, the effects of this ceRNET on cell apoptosis and related mechanisms remain unclear. In the present study, we found that downregulation of CYP4Z1 or the CYP4Z2P 3'-UTR promotes cell apoptosis, mirroring the functions of human telomerase reverse transcriptase (hTERT). Furthermore, the ceRNET between CYP4Z1 and pseudogene CYP4Z2P modulates hTERT expression by operating as a sub-ceRNET for hTERT. Our data demonstrate that the ceRNET between CYP4Z1 and pseudogene CYP4Z2P acts as a sub-ceRNET for hTERT and, thus, inhibits breast cancer apoptosis.

• Authors: Cheng Li, Lufeng Zheng, Yu Xin, Zhoulin Tan, Yan Zhang, Xia Meng, Zhigang Wang, Tao Xi
• Journal: FEBS letters (FEBS Lett) Vol. 591 Issue 7 Pg. 991-1000 (Apr 2017) ISSN: 1873-3468 [Electronic] England
• PMID: 28236635 (Publication Type: Journal Article)
• Copyright: © 2017 Federation of European Biochemical Societies.

Chemical References

• 3' Untranslated Regions
• MIRN125 microRNA, human
• MicroRNAs
• RNA, Neoplasm
• CYP4Z1 protein, human
• Cytochrome P450 Family 4
• TERT protein, human
• Telomerase

Topics

• 3' Untranslated Regions (genetics)
• Apoptosis (genetics)
• Base Sequence
• Blotting, Western
• Breast Neoplasms (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cytochrome P450 Family 4 (genetics, metabolism)
• Down-Regulation
• Female
• Gene Expression Regulation, Neoplastic
• HEK293 Cells
• Humans
• MCF-7 Cells
• MicroRNAs (genetics)
• Pseudogenes (genetics)
• RNA, Neoplasm (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Homology, Amino Acid
• Telomerase (genetics, metabolism)